1. Simple column-switching ion chromatography method for determining eight monosaccharides and oligosaccharides in honeydew and nectar.
- Author
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Ni, Chengzhu, Zhu, Binhe, Wang, Nani, Wang, Muhua, Chen, Suqing, Zhang, Jiajie, and Zhu, Yan
- Subjects
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MONOSACCHARIDES , *ION exchange chromatography , *OLIGOSACCHARIDES , *NECTAR , *SUGAR , *STANDARD deviations - Abstract
Honeydew is excreted by aphids as a sweet waste and nectar is floral honey. Honeydew and nectar are complicated samples which consist of various sugars and amino acids. In this work, a simple ion chromatography with column-switching method was developed for the simultaneous analysis of 8 monosaccharides and oligosaccharides in honeydew and nectar. A reversed-phase column was used as a pretreatment column to eliminate organics on-line and sugars were eluted from a collection loop to analytical column by using column-switching technique. This method showed good linearity ( r ⩾ 0.9994) and afforded low limits of detection ranging from 1.55 to 10.17 μg L −1 for all the analytes. Recoveries ranged from 95% to 105% and repeatability results were acceptable with relative standard deviation of less than 3.21% ( n = 6). This method was successfully applied to quantification of these sugars in honeydew and nectar. These results showed honeydew had much more oligosaccharides than nectar. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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