1. Can sucrose-substitutes increase the antagonistic activity against foodborne pathogens, and improve the technological and functional properties of sheep milk kefir?
- Author
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Larosa CP, Balthazar CF, Guimarães JT, Margalho LP, Lemos FS, Oliveira FL, Abud YKD, Sant'Anna C, Duarte MCKH, Granato D, Raices RSL, Freitas MQ, Sant'Ana AS, Almeida Esmerino E, Pimentel TC, Cristina Silva M, and Cruz AG
- Subjects
- Animals, Antioxidants chemistry, Cell Line, Cell Proliferation drug effects, Humans, Hydrogen-Ion Concentration, Kefir microbiology, Kefir toxicity, Lactobacillus isolation & purification, Lactobacillus metabolism, Lactococcus isolation & purification, Lactococcus metabolism, Milk chemistry, Peptidyl-Dipeptidase A chemistry, Peptidyl-Dipeptidase A metabolism, Principal Component Analysis, Sheep, Volatile Organic Compounds analysis, Yeasts isolation & purification, Yeasts metabolism, alpha-Amylases antagonists & inhibitors, alpha-Amylases metabolism, Kefir analysis, Sucrose chemistry
- Abstract
The effect of different types of sugar (sucrose, demerara, brown, fructose, coconut sugar, and honey) on sheep milk kefir was evaluated. Microbial counts (Lactobacillus, Lactococcus, Leuconostoc, yeast), antagonistic activity against foodborne pathogens, microstructure (scanning electron microscopy), and antiproliferative activity of cancer cells were evaluated. Furthermore, the antioxidant activity (DPPH), inhibitory activity of angiotensin-converting enzyme (ACE), α-amylase, and α-glucosidase, lactose content, lactic and acetic acids and ethanol, fatty acid profile and volatile organic compounds were determined. The addition of sugars increased the Lactobacillus population (up to 2.24 log CFU/mL), metabolites concentration, antagonistic activity against pathogens, antioxidant activity (11.1 to 24.1%), ACE inhibitory activity (27.5 to 37.6%), α-amylase inhibition (18 to 37.4%), and anti-proliferative activity. Furthermore, it improved the fatty acid profile and volatile compounds. The results suggest that the replacement of sucrose with different types of sugar constitute an interesting option to be used in sheep milk kefir formulations., (Copyright © 2021 Elsevier Ltd. All rights reserved.)
- Published
- 2021
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