1. Exogenous application of phytosulfokine α (PSKα) delays yellowing and preserves nutritional quality of broccoli florets during cold storage
- Author
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Morteza Soleimani Aghdam, Zisheng Luo, and Mohammad Sayyari
- Subjects
Chalcone synthase ,Cold storage ,Guanosine ,Phenylalanine ,Ascorbic Acid ,Brassica ,01 natural sciences ,Analytical Chemistry ,chemistry.chemical_compound ,0404 agricultural biotechnology ,Phenols ,Gene expression ,Food Quality ,Plant Proteins ,Oxidase test ,biology ,Chemistry ,Pigmentation ,Phytosulfokine ,010401 analytical chemistry ,food and beverages ,04 agricultural and veterinary sciences ,General Medicine ,Ascorbic acid ,040401 food science ,0104 chemical sciences ,Cold Temperature ,Horticulture ,Food Storage ,biology.protein ,Nutritive Value ,Food Science - Abstract
In this study, we tested the exogenous application of phytosulfokine α (PSKα) for delaying the yellowing of broccoli florets during cold storage. Our results showed that the lower yellowing in broccoli florets treated with 150 nM PSKα was probably due to the higher endogenous accumulation of PSKα, leading to the endogenous accumulation of guanosine 3′, 5′-cyclic monophosphate (cGMP). Besides, broccoli florets treated with 150 nM PSKα exhibited a higher accumulation of phenols and flavonoids by triggering gene expression and activities of phenylalanine ammonia-lyase (PAL) and chalcone synthase (CHS). Moreover, the higher expression of L‐galactotno‐1,4‐lactone dehydrogenase (GLDH) gene and the lower expression of ascorbic acid oxidase (AAO) gene in broccoli florets treated with 150 nM PSKα may be the reasons for the higher accumulation of ascorbic acid. In conclusion, the exogenous application of PSKα is a promising strategy in delaying the yellowing and preserving the nutritional quality of broccoli florets during cold storage.
- Published
- 2020