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4. Improving the freeze-drying survival rate of Lactobacillus plantarum LIP-1 by increasing biofilm formation based on adjusting the composition of buffer salts in medium

Author

Ma Rongze, E. Jingjing, Chen Zichao, Sun Ruiyin, Yao Caiqing, Wang Ruixue, He Zongbai, Wang Junguo, and Zhang Qiaoling

Subjects
Salt (chemistry), Buffers, 01 natural sciences, Analytical Chemistry, Lactones, Freeze-drying, chemistry.chemical_compound, 0404 agricultural biotechnology, Bacterial Proteins, Homoserine, Food science, chemistry.chemical_classification, Microbial Viability, Dose-Response Relationship, Drug, biology, 010401 analytical chemistry, Biofilm, Quorum Sensing, food and beverages, 04 agricultural and veterinary sciences, General Medicine, biochemical phenomena, metabolism, and nutrition, biology.organism_classification, 040401 food science, Culture Media, 0104 chemical sciences, Lactic acid, Survival Rate, Carbon-Sulfur Lyases, Quorum sensing, Freeze Drying, Real-time polymerase chain reaction, chemistry, Biofilms, Salts, Lactobacillus plantarum, Bacteria, Food Science
Abstract

Lactic acid bacteria can improve their resistance to adverse environments through the formation of biofilm. This study found that adding different buffer salts in culture medium had a great impact on the freeze-drying survival rate of the Lactobacillus plantarum LIP-1, which could be linked to biofilm formation. Transcriptome data showed that potassium ions in buffer salt increased the expression of the luxS gene in the LuxS/autoinducer-2 (AI-2) quorum sensing system and increase synthesis of the quorum sensing signal AI-2. The AI-2 signal molecules up-regulated the cysE gene, which helps to promote biofilm formation. By adding a biofilm inhibitor, d-galactose, and performing a real-time quantitative polymerase chain reaction experiment, we found that d-galactose could down-regulated the luxS and cysE genes, reduced biofilm formation, and decreased the freeze-drying survival rate. The results of this study showed that promoting biofilm formation using appropriate buffer salts may lead to better freeze-drying survival rates.

Published
2021

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