1. Identification of phenolic compounds in red wine extract samples and zebrafish embryos by HPLC-ESI-LTQ-Orbitrap-MS.
- Author
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Vallverdú-Queralt, Anna, Boix, Nuria, Piqué, Ester, Gómez-Catalan, Jesús, Medina-Remon, Alexander, Sasot, Gemma, Mercader-Martí, Mercè, Llobet, Juan M., and Lamuela-Raventos, Rosa M.
- Subjects
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PHENOL analysis , *RED wines , *HIGH performance liquid chromatography , *MASS spectrometry , *ZEBRA danio , *EMBRYOS - Abstract
The zebrafish embryo is a highly interesting biological model with applications in different scientific fields, such as biomedicine, pharmacology and toxicology. In this study, we used liquid chromatography/electrospray ionisation-linear ion trap quadrupole-Orbitrap-mass spectrometry (HPLC/ESI-LTQ-Orbitrap-MS) to identify the polyphenol compounds in a red wine extract and zebrafish embryos. Phenolic compounds and anthocyanin metabolites were determined in zebrafish embryos previously exposed to the red wine extract. Compounds were identified by injection in a high-resolution system (LTQ-Orbitrap) using accurate mass measurements in MS, MS 2 and MS 3 modes. To our knowledge, this research constitutes the first comprehensive identification of phenolic compounds in zebrafish by HPLC coupled to high-resolution mass spectrometry. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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