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Street foods are often of poor nutritional quality with high sugar content, in which the overconsumption of sugar is associated with obesity. However, sugar content information on local street foods is scarce. Thus, the individual and total sugar contents of 94 types of street foods in Malaysia were analysed. Compared to snacks and main meals, desserts contained the highest amounts of sugar, sucrose, fructose, glucose, and maltose. Sucrose was predominant in 90% desserts, 79.3% snacks, and 68.6% main meals. Most desserts (93.3%) contained medium to high sugar content (≥5 g to >15 g/100 g), while 82.9% main meals and 65.5% snacks had low sugar content. When comparing the sugar contents of 39 foods with other local databases, 58.3% main meals, 55.6% desserts, and 33.3% snacks contained either significantly (p < 0.05) higher or lower sugar contents. Consumers can identify low and high-sugar foods, and policymakers can review health priorities to combat obesity. • Individual and total sugar contents in main meals, desserts, and snacks. • Most desserts contained medium to high sugar, predominantly contributed by sucrose. • Most main meals and snacks contained low sugar, and mostly contributed by sucrose. [ABSTRACT FROM AUTHOR]

Maple syrup, a popular natural sweetener has a high content of sucrose, whose consumption is linked to different health issues such as obesity and diabetes. Hence, within this paper, the conversion of sucrose to prebiotics (fructo-oligosaccharides, FOS) was proposed as a promising approach to obtaining a healthier, value-added product. Enzymatic conversion was optimized with respect to key experimental factors, and thereafter derived immobilized preparation of fructosyltransferase (FTase) from Pectinex® Ultra SP-L (FTase-epoxy Purolite, 255 IU/g support) was successfully utilized to produce novel functional product in ten consecutive reaction cycles. The product, obtained under optimal conditions (60 °C, 7.65 IU/mL, 12 h), resulted in 56.0% FOS, 16.7% sucrose, and 27.3% monosaccharides of total carbohydrates, leading to a 1.6-fold reduction in caloric content. The obtained products' prebiotic potential toward the probiotic strain Lactobacillus plantarum 299v was demonstrated. The changes in physico-chemical and sensorial characteristics were esteemed as negligible. [Display omitted] • Maple syrup modification to value-added product with prebiotic potential. • In situ enzymatic transformation of sucrose to fructo-oligosaccharides (FOS). • Decrease in sucrose concentration (82%) led to a 1.6-fold reduction in caloric value. • Product retained original physico-chemical and sensorial properties. • Product remains stable for at least one year without FOS degradation. [ABSTRACT FROM AUTHOR]

C-Phycocyanin and sugar (C-PC/S) blended agar hydrocolloid was prepared and its rheological, thermo-functional and morphological properties were examined based on the fluorescence excitation-emission matrix profile. Sucrose (40%, w / v) determined as a superior preservative, maintaining the native conformation of C-PC effectively. C-PC/S exhibited enhanced structural integrity with high storage modulus (G') and 86.4% swelling index. FT-IR demonstrated strong intramolecular bonding. TGA revealed that the presence of sucrose prolonged the devolatilization peak up to 325 °C, with a degradation rate of −2.273 mg/min, it the thermal stability. C-PC/S fortified hydrocolloid in ice cream (5.0% w /w), reduced melting rate up to five times. In conclusion, sucrose as a promising enhancer of color stability and structural integrity for C-PC, and this combination effectively improves the functional and rheological properties. Further, the findings exposed the agar hydrocolloid as a potential enhancer of color retention and improved performance for various food and cosmetic products. [Display omitted] • The study focused on the problems with colour preservation and thermal stability when employing C-Phycocyanin (C-PC) as a natural colorant in the food industries. Our research created a C-PC and sucrose (C-PC/S) blended agar hydrocolloid and looked at its qualities to address these issues. • Sucrose at a concentration of 40% (w / v) was found best among the other additions evaluated, preserving C-PC in its natural structure and improving colour stability. Hence, sucrose as a superior preservative according to our study report. • Enhanced Structural stability and integrity: The C-PC/S blended agar hydrocolloid exhibited improved structural integrity and stability with high storage module (G') and a significant swelling index (86.4%). • Strong intramolecular bonding: FTIR report revealed strong intramolecular bonding between C-PC, sucrose and agar polymer, it contributes stability of the colourant. • Thermal stability improved: The presence of sucrose in the agar hydrocolloids extended the devolatilization peak up to 325oC and improved thermal stability, it withstands prolonged temperature. • Application as a colouring agent in ice cream: C-PC/S fortified hydrocolloids in ice cream at 5.0 % w /w reduced the melting rate up to five times, so it is a promising natural colourant for frozen food products. • Overall, the study highlighted with many findings i.e. sucrose as promising enhancer of colour stability and structural integrity for C-PC, by which it effectively improved the functionality and rheological features. Therefore, the blended C-PC/S based agar hydrocolloid provides a possible solution to address colour retention and stability challenges in various food products making industry as an attractive green initiative. [ABSTRACT FROM AUTHOR]

Reactive carbonyl species can modify digestive enzymes upon intake due to their electrophilic nature. This study evaluated the effects of methylglyoxal (MGO), glyoxal, acrolein, and formaldehyde on invertase, an enzyme presents in digestive tract. Unexpectedly, MGO enhanced, rather than inhibited, invertase activity. Moreover, MGO counteracted the inhibitory effects of the other three carbonyls on invertase activity. Kinetic analyses revealed that 150 mmolLexp.-1 MGO resulted in a 2-fold increase in the Km and a 3.3-fold increase in Vmax, indicating that MGO increased the turnover rate of sucrose while reducing the substrate binding affinity of invertase. Additionally, MGO induced dynamic quenching of fluorescence, reduced free amino groups, increased hydrophobicity, the content of Amadori products, fluorescent and nonfluorescent AGEs, and amyloid fibrils of invertase. The specific modifications responsible for the elevated activity of MGO on invertase require further investigation. • Methylglyoxal (MGO) increases the activity of invertase. • MGO reverses the inhibitory effect of other three carbonyl compounds. • MGO caused a 2-fold increase in K m and 3.3-fold increase in V max. • MGO modified invertase and may change the enzyme structure. [ABSTRACT FROM AUTHOR]

To improve the stability of pollock bone broth, compound emulsifiers were employed and evaluated in nano-emulsions from pollock bones (PBNs). The microstructure, creaming index, particle size, zeta potential, and viscosity of PBNs were characterized and the stability of PBNs was investigated. It revealed that the concentration of compound emulsifiers is one of the principal factors for particle size, zeta potential, and viscosity of PBNs, and 0.9% of sodium caseinate and sucrose fatty acid ester (CS-SE) can make the PBN display good stability. Its particle size changed from 81.17 ± 1.33 nm to 19.62 ± 0.21 nm when the temperature ranged from 40 °C to 80 °C, and its creaming index could reach a maximum (90.83%) among all PBNs in 4 months of freeze-thaw assays. PBN stability could be improved by the compound emulsifier (CS-SE), which offers a theoretical basis for the application of pollock bone broth. • Oil-in-water nano-emulsions from pollock bones (PBNs) were fabricated. • Compound emulsifiers on the properties of PBNs were investigated. • The particle sizes of PBNs decreased as emulsifiers' concentration increased. • The thermal and freeze-thaw stability of the PBNs were evaluated. • PBN of 0.9% CS-SE was relatively uniform and stable. [ABSTRACT FROM AUTHOR]

Turanose, an isomer of sucrose, naturally exists in honey. Previous study indicated that turanose content increased gradually in acacia honey as honeybees brewed honey in the hive. However, it is unclear how turanose is generated in honey. We hypothesised that turanose was produced by enzymes from honeybees and performed a series of simulation experiments to prove this hypothesis. We found turanose in honey was produced by honeybees processing sucrose. Furthermore, we determined that sugar composition of simulated nectar influenced the turanose concentration in honey: when sucrose concentration was below 5%, turanose was difficult to form, whereas high concentration of fructose and limited glucose were beneficial in producing turanose. Using 13C-labelled sucrose tests combined with proteomics analysis, we identified that α-glucosidase converted sucrose to turanose through an intermolecular isomerisation process. This study reveals the formation mechanism of turanose in honey and assists in the scientific control and improvement of honey quality. [Display omitted] • Formation mechanism of turanose in honey was explored. • Sucrose was confirmed as the substrate to form turanose. • Enzyme was identified as α-glucosidase. • Turanose was formed via an intermolecular isomerization process. [ABSTRACT FROM AUTHOR]

The characteristic aroma compounds of traditional braised pork were investigated by gas chromatography–mass spectrometry−olfactometry (GC–MS–O), odor-activity values, and aroma recombination and omission experiments. A total of 56 volatile compounds were detected by GC–MS, among which hexanal, octanal, nonanal, (E)-2-octenal, 2,3-octanedione, 1-octen-3-ol, 2-pentylfuran, methanethiol, and dimethyl trisulfide were identified as the key aroma compounds by molecular sensory science. Partial least squares regression analysis indicated that some aroma compounds significantly contributed to fatty (hexanal, heptanal, 2-pentylfuran, nonanal, and (E)-2-octenal), meaty (methanethiol, dimethyl disulfide, dimethyl trisulfide, and octanal), sauce-like flavor (3-hydroxy-2-butanone and 2-furfural), and sweet, caramel (2,3-octanedione, 1-octen-3-ol). Lean meat produced more aldehydes, alcohols, ketones, and sulfur-containing compounds than subcutaneous fat. The seasonings (saccharose, cooking wine, and soy sauce) facilitated the formation of ethyl L-lactate, 2-acetylfuran, 2-furfural, 5-methyl-2-furaldehyde, 2-methyl-pyrazine, and 2-acetylpyrrole. Meanwhile they reduced the content of lipid oxidation products, thereby stimulated the characteristic aroma of the Chinese traditional braised pork. [Display omitted] • Key aroma compounds in braised pork were identified by molecular sensory science. • PLSR analysis revealed correlation between aroma compounds and sensory quality. • PLSR analysis can serve as complementary method of molecular sensory science. • Lean meat produced more aroma compounds than subcutaneous fat. • Seasonings improved flavor profile by weakening excessive lipid oxidation. [ABSTRACT FROM AUTHOR]

Phenylalanine (Phe) accelerates fruit wound healing by activating phenylpropanoid metabolism. However, whether Phe affects sucrose and respiratory metabolism in fruit during wound healing remains unknown. In this research, we found that preharvest Phe spray promoted sucrose degradation and increased glucose and fructose levels by activating acid invertase (AI), neutral invertase (NI), sucrose synthase (SS) and sucrose phosphate synthase (SPS) on harvested muskmelons. The spray also activated hexokinase (HK), phosphofructokinase (PFK), pyruvate kinase (PK), malate dehydrogenase (MDH), succinate dehydrogenase (SDH) and glucose-6-phosphate dehydrogenase (G6PDH). In addition, the spray improved energy and reducing power levels in the fruit. Taken together, preharvest Phe spray can provide carbon skeleton, energy and reducing power for wound healing by activating the sucrose metabolism, Embden-Meyerhof-Parnas (EMP) pathway, tricarboxylic acid (TCA) cycle and pentose phosphate (PPP) pathway in muskmelon wounds during healing, which is expected to be developed as a new strategy to accelerate fruit wound healing. • Preharvest Phe sprays activated sucrose metabolism at wounds in muskmelons. • Preharvest Phe sprays activated the EMP pathway and the TCA cycle in wounds. • Preharvest Phe sprays activated the PPP pathway at wounds. • Preharvest Phe sprays increased energy levels and reducing power at wounds. [ABSTRACT FROM AUTHOR]

• High osmotic pressure system facilitated the preparation of reduced-salt salted egg yolk. • The mixed sucrose-phosphate-salt pickling system accelerated salt penetration. • Phosphate promoted the formation of dense and stable network structures in protein. • Small molecular weight protein bound with each other to form large aggregates. • The salted egg yolk of 28 d had loose sandy texture and lower salt content (1.07%). This paper was dedicated to the study of the effect of sucrose-phosphate on aspects of physicochemical properties, lipid distribution and protein structure during the picklig of reduced‐salt salted egg yolk (SEY). This work constructed a reduced‐salt pickling system from a new perspective (promoting osmosis) by using a sucrose-phosphate-salt. Results showed that SEY-28d achieved a desirable salt content (1.07 %), hardness (573.46 g) and springiness (0.65 g). The matured SEY was in excellent quality with orange-red color and loose sandy texture. This was because the lipoprotein aggregated with each other through hydrophobic interaction to form a stable network structure. In addition, the hypertonic environment accelerated salt penetration. These also created good condition for lipid spillage. The results of confocal laser scanning microscope also verified this phenomenon. This work provides important guidance for new reduced‐salt curing of traditional pickled foods, deep processing of SEY, and industry development in the field of poultry egg. [ABSTRACT FROM AUTHOR]

• The metabolic pathway networks of three energy substances in flaxseed germination were proposed. • Arginine and Proline metabolism were the key pathways in flaxseed during germination. • During flaxseed germination, arginine was the most active metabolite. • Sucrose partakes in galactose metabolism and starch and sucrose metabolism. • The main phospholipid molecules in the flaxseed were PA, PE, LPA, and LPC at 0–7 d. Germinated flaxseed (Linum usitatissimum L.) is an essential potential food ingredient, but the major energy substances (proteins, lipids, and carbohydrates) metabolites and metabolic pathways are unknown. Comprehensive metabolomic analyses were performed using Fourier transform infrared spectroscopy and high-performance liquid chromatography mass spectrometry on flaxseed from 0 to 7 d. Additionally, the critical metabolites pathways networks of three energy substances metabolites during flaxseed germination were exhibited. The results showed that arginine was the most active metabolite during germination, strongly associated with the arginine biosynthesis and arginine and proline metabolism pathways. Carbohydrates predominantly comprised sucrose on 0–3 d, which participated in galactose metabolism and starch and sucrose metabolism. The main flaxseed phospholipid molecules were phosphatidic acid, phosphatidylethanolamine, lysophosphatidic acid, and lysophosphatidylcholine during germination. This study underscores the paramount metabolic pathways in proteins, lipids and carbohydrates were arginine and proline metabolism, linoleic acid metabolism, arachidonic acid metabolism, and ascorbate and aldarate metabolism during germination. [ABSTRACT FROM AUTHOR]

[Display omitted] • Construction of a sugar-smoking model reaction to study the primary pathways of sugar-smoking pigment formation. • The primary source of 5-HMF was identified as fructose resulting from the cleavage of sucrose at 330 °C. • The primary pathway for the formation of 5-GGMF was attributed to the intermolecular dehydration of 5-HMF and glucose at 150 °C. • The 5-HMF-glucose model reaction product displayed stronger radical scavenging abilities. The present study aimed to elucidate the pathway of pigment formation and identify the source of antioxidant activity during sugar smoking. Building upon previous research, this investigation replicated the sucrose cleavage process involved in sugar-smoking through model reactions to obtain distinct model reaction products. The products were analyzed using various techniques such as ultraviolet–visible spectrometry, Fourier-transform infrared spectroscopy, high-performance liquid chromatography, and high-performance liquid chromatography-tandem mass spectrometry. The findings revealed that the pyrolysis of sucrose at 330 °C yielded glucose and fructose, with fructose pyrolysis producing significantly more 5-HMF than glucose. Moreover, the antioxidant capacity of 5-HMF was found to make a substantial contribution. The primary source of 5-HMF was identified as fructose resulting from the cleavage of sucrose at 330 °C, while the primary pathway for the formation of the sugar-smoking pigment 5-GGMF was attributed to the intermolecular dehydration of 5-HMF and glucose at 150 °C. [ABSTRACT FROM AUTHOR]

• Combination of citric acid addition with heating treatment intensified color density. • Addition of citric acid increased antioxidant activity in capulin extracts. • Main anthocyanin in capulin extracts was malvidin 3-O-galactoside. • Major non-anthocyanin polyphenol in capulin extracts was myricetin-O-hexoside. The objective of this study was to analyze and optimize the influence of heating time and citric acid (CA) or sucrose addition of Ardisia compressa K. extracts on phenolic compounds (TPC), monomeric anthocyanins (MAA), antioxidant activity (TAC), color density (CD), and hue tint (HT), using a full factorial design. Extractions were performed: temperature (25, 50, or 70 °C), time (15, 30, 60, or 90 min), CA (0.0 or 0.02 g), and sucrose (0.0 or 5.0 g). HPLC-DAD-ESI-MS was conducted in extracts without additives and with the addition of CA (0.02 g) or sucrose (5.0 g), at 25, 50, or 70 °C for 15 min. CA-added extracts showed maximum TPC, MAA, TAC (DDPH and ABTS assays), and CD values, with the lowest HT values. Malvidin 3- O -galactoside and myricetin- O -hexoside were the predominant anthocyanin and non-anthocyanin polyphenols. Time, temperature, and solute influenced the optimized extraction of TPC, MAA, anthocyanins, TAC, CD, and HT. [ABSTRACT FROM AUTHOR]

The physicochemical and foam properties of non-purified water extracts (WE) and purified tea saponins (TS) from Camellia oleifera cake (byproduct) were compared. WE showed different fluid properties at equal saponin concentrations (1.0 wt%) compared to TS. Particularly, it exhibited limited micelle size (average 434.1 nm), effective viscosity (0.15 Pa·s), and surface tension (43.9 mN/m) independently of pH. Moreover, the foam properties of WE were comparable to TS and better than sodium caseinate, especially foam stability. WE foam was more stable than TS foam under pH (3-7) and heating (40-80 °C). In the presence of NaCl, sucrose, and ethanol (5-20 wt%), WE and TS were effective and had similar foam behavior. Low concentrations of sucrose (<10 wt%)/ethanol (<20 wt%) significantly increased the foam capacity, while ethanol over 30 wt% was unfavorable. WE/TS foam contributes significantly to the desired physicochemical and sensory attributes (taste, texture, and appearance) of foods., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

• CVTC could better maintain the quality of sweet potato tuberous roots; • CVTC regulate starch degradation-related enzymes (α-amylase and β-amylase); • CVTC activated sucrose synthesis-related enzymes; • CVTV has great potential to enhance the sweetness of tuberous roots. This study aimed to investigate the influence of cyclic variable temperature conditioning (CVTC) on the rapid sweetening of sweet potato tuberous roots, as assessed through the analysis of sugar metabolism-related compounds and enzyme activities of tubers during storage. The results showed that CVTC effectively preserved the quality of sweet potato tuberous roots, leading to a significant elevation in soluble solids and soluble sugars. The CVTC group displayed sucrose and fructose levels that were 1.72 and 1.46 times higher, respectively, compared to the control group at the 8 d. Additionally, after storage, the activities of β-amylase, sucrose phosphate synthase (SPS), and sucrose synthase (SS) in the CVTC group were increased by 19.85 %, 60.74 %, and 82.48 %, respectively. Conversely, acid convertase (AI) activity showed inhibition of 64.72 %. In conclusion, implementing CVTC enhanced enzymatic activity in β-amylase, SPS, and SS, facilitating starch degradation and sucrose synthesis, which contributed to the overall improvement in the sweetness of sweet potato tubers. [ABSTRACT FROM AUTHOR]

Fructan, a widespread functional polysaccharide, has been used in the food, pharmaceutical, cosmetic, and material production fields because of its versatile physicochemical properties and biological activities. Inulin from plants and levan from microorganisms are two of the most extensively studied fructans. Fructans from different plants or microorganisms have inconsistent molecular weights, and the molecular weight of fructan affects its properties, functions, and applications. Recently, increasing attention has been paid to the production and application of fructans having various molecular weights, and biotechnological processes have been explored to produce tailor-made fructans from sucrose. This review encompasses the introduction of extraction, enzymatic transformation, and fermentation production processes for fructans with diverse molecular weights. Notably, it highlights the enzymes involved in fructan biosynthesis and underscores their physiological effects, with a special emphasis on their prebiotic properties. Moreover, the applications of fructans with varying molecular weights are also emphasized., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Elsevier Ltd. All rights reserved.)

[Display omitted] • Allulose and kestose were applied as a sugar alternative in a cookie system. • Kestose led to lower water mobility than allulose, delaying starch gelatinization. • Delayed gelatinization lowered pasting/thermo-mechanical properties of wheat flour. • Kestose was more feasible to replace sucrose in terms of cookie quality attributes. The effects of different sweeteners on the physicochemical properties of soft wheat flour were investigated mainly in terms of thermal, rheological, and water mobility features, and their feasibilities as an alternative to sugar were evaluated in the cookie system. Kestose significantly reduced the solvent retention capacity of wheat flour, followed by sucrose, fructose, and allulose. Thermal analysis showed that the sucrose and kestose distinctly led to an increase in the gelatinization temperature of wheat flour, which was explained by lower T 2 relaxation times. In addition, the pasting viscosities and thermo-mechanical properties of wheat flour containing kestose became lower compared to allulose, and these differences were morphologically confirmed by the real-time microscopic measurements during heating. Furthermore, when the sweeteners were incorporated into the cookie formulations, kestose played a positive role as a sugar replacer in the cookie system by presenting a comparable spread factor, texture, and color to cookies with sucrose. [ABSTRACT FROM AUTHOR]

• Low temperature slow down the loss of nutrition and maintain quality of sweet corn kernels. • A total of 776 DAMs were obtained in sweet corn kernels during storage. • DAMs were enriched in flavonoid biosynthesis, linoleic acid metabolism and sphingolipid metabolism. • Ten metabolites could be potential biomarkers for identifying sweet corn kernels at low temperature during storage. Fresh sweet corn has a limited shelf-life due to its high moisture and high sugar content. Low temperature storage is an effective technique employed to extend the shelf-life. However, changes in the chemical composition of sweet corn kernels at low temperatures are not fully understood. In this study, kernels stored at low temperature exhibited higher levels of soluble sugars and lower starch content. In total, 1365 metabolites were characterized in sweet corn kernels. 593 and 308 differentially accumulated metabolites (DAMs) were identified in sweet corn kernels stored at normal and low temperature, respectively. 607 DAMs were identified at low temperature compare to normal temperature. DAMs were consistently enriched in flavonoid biosynthesis, linoleic acid metabolism and sphingolipid metabolism. Moreover, dozens of metabolites were identified as potential biomarkers for post-harvest storage effects in sweet corn. These results extend our knowledge of the dynamic changes in sweet corn kernels stored at low temperatures. [ABSTRACT FROM AUTHOR]

[Display omitted] • Improved discrimination of sugar sources by 1H NMR using MCR-ALS as preprocessing. • Reliable identification of compounds for sugar differentiation from MCR-ALS outputs. • Discrimination of brown edible sugars attributed to organic acids and carbohydrates. • Discrimination of sugar beet by origin and storage attributed to amino acids. • MCR-ALS allows the differentiation of highly similar 1H NMR spectra of sugars. The different types of sugar employed in the food industry exhibit chemical similarity and are mostly dominated by sucrose. Owing to the sugar origin of and differences in production, the presence of certain minor organic compounds differs. To differentiate between sugars based on their botanical source, geographical origin, or storage conditions, commercial brown sugars and sugar beet extracts were analyzed by 1H NMR spectroscopy applying a segmented analysis by means of multivariate curve resolution-alternating least squares (MCR–ALS). Principal component analysis and partial least squares-discriminant analysis yielded excellent differentiation between sugars from different sources after the application of this preprocessing strategy; without loss of chemical information and with direct interpretation of the results. By applying a segmented analysis via MCR–ALS to 1H NMR sugar data, similar spectroscopic profiles could be differentiated. This improved the selectivity of 1H NMR spectroscopy for sugar source differentiation which can be useful for industrial sugar authentication purposes. [ABSTRACT FROM AUTHOR]

This study aims to evaluate the effect of sucrose replacer mixtures (erythritol, mannitol, or tagatose in combination with inulin or polydextrose) on the crystal morphology, particle size distribution, rheology, melting properties, and fat polymorphism of dark compound chocolate. The result showed that the replacer mixture's hygroscopicity, particle size, and sugar crystal shape might significantly impact dark compound chocolate's rheological and textural properties but had no substantial impact on the melting properties and fat crystallization. Mannitol-containing samples exhibited the highest rheological value, likely related to their high moisture content, small particle size, and elongated crystal shape. Due to the similar specific surface area and comparable D90 value, the sample containing erythritol-polydextrose mixture resulted in a similar (P ≥ 0.05) Casson yield value (46.184 ± 2.45 Pa) compared to the sample containing sucrose (38.348 ± 1.68 Pa). It could be a potential sucrose replacer in the dark compound chocolate., Competing Interests: Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2023 Monash University Malaysia. Published by Elsevier Ltd.. All rights reserved.)

• Thermophysical data were measured for {sucrose + ionic liquid + water} solutions. • Taste behavior of sucrose was studied by computing ASV and ASIC parameters. • Both ionic liquids show dehydration effect on aqueous sucrose solutions. • The ionic–hydrophilic interactions are dominant in these systems. • Δ tr V Φ 0 , Δ tr K Φ 0 and Δ tr B increase by enhancement in the ionic liquid concentration. The thermophysical properties and taste behavior of sucrose in aqueous ionic liquids {1-octyl-3-methyl imidazolium bromide and 1-octyl-3-methyl imidazolium chloride} solutions have been studied via volumetric, compressibility and viscometric properties at T = (288.15, 298.15, 308.15 and 318.15) K. The positive values of transfer volume and transfer partial molar isentropic compression indicated predominance of ionic–hydrophilic interactions in these systems. The calculated hydration number for sucrose demonstrated that dehydration of sucrose occurs in presence of both studied ionic liquids. Further, the effect of studied ionic liquids on the taste behaviour of sucrose has been discussed from ASV and ASIC parameters. Furthermore, interaction volume, V int , and cavity volume, V cav , have been computed from scaled particle theory (SPT). [ABSTRACT FROM AUTHOR]

• Lower brew global scores were linked to high caffeine, protein and CGA contents. • High sucrose/acid and cafestol/kahweol ratios in the bean indicate high-quality brew. • High cafestol content in the bean was also associated with high brew global score. This research aimed to correlate the composition of green Arabica coffee beans with the sensory quality coffee brews. The chemical composition of green Arabica coffee bean (66 samples) from three coffee quality contests was analyzed by near-infrared spectroscopy. Coffee brews with lower quality scores were correlated with high levels of caffeine, protein, chlorogenic acids and total titratable acidity (TTA) in the green coffee beans. High sucrose/TTA and cafestol/kahweol ratios in the green coffee beans were usually associated with higher scores for the coffee brews. By multivariate analysis techniques, the samples were separated into groups according to production years indicating a strong influence of the environmental conditions on the chemical composition. The profile of the composition of the crude coffee can be indicative of the sensory quality of the coffee brews, relevant information for producers and industry since the green beans are the material used for trading and purchasing coffee. [ABSTRACT FROM AUTHOR]

Highlights • Curcumin-containing proliposomes can be produced by coating of micronized sucrose. • Nonpurified phospholipids are suitable to produce proliposomes. • Curcumin was efficiently protected when encapsulated in liposome dispersions. • Amount of nonpurified phospholipids had a crucial role on liposome stability. • Mixed curcumin-loaded vesicles remained stable for a short storage time. Abstract The feasibility of producing proliposomes containing curcumin, as well as liposome dispersions, using different mixtures of purified and nonpurified soybean phospholipids was studied. Proliposomes were produced through coating of micronized sucrose and physicochemically characterized over 30 days of storage. In addition, the possible interactions among the components were investigated using X-ray powder diffraction (XRD) and Fourier transform infrared spectroscopy (FT-IR). The proliposomes demonstrated a low propensity of water adsorption and low hygroscopicity. In addition, the curcumin content retained in the powders ranged from 67 to 92%. The liposomes were produced following proliposome hydration. Atomic force microscopy indicated the vesicles presented spherical shapes and photon correlation spectroscopy detected that their hydrodynamic diameters ranged from 207 to 222 nm. Finally, the curcumin-loaded liposomes preserved up to 63% of the bioactive compound but remained stable for only 15 days of storage. [ABSTRACT FROM AUTHOR]

Highlights • NFT storage did not result in visual CI symptoms in nectarine fruit. • NFT reduced sucrose degradation to enhance chilling tolerance in nectarine fruit. • NFT-stored fruit showed higher activities of HXK and FRK. • Chilling resistance in NFT-stored fruit was related to higher energy status. Abstract To avoid chilling injury (CI) of nectarines during storage, the impact of near-freezing temperature (NFT) (−1.4 ± 0.1 °C), 0 ± 0.1 °C and 5 ± 0.1 °C on CI incidence, ion leakage, levels of soluble sugars and enzymatic activities related to soluble sugars and energy metabolism, were investigated over five weeks. NFT-stored fruit showed no CI symptoms and significantly (P < 0.05) lower increase of ion leakage than those kept at 0 and 5 °C. NFT significantly (P < 0.05) diminished the activities of sucrose metabolism-associated enzymes leading to a higher level of sucrose in fruit, and maintained higher activities of hexokinase and fructokinase. Additionally, NFT-stored fruit exhibited significantly (P < 0.05) higher activities of energy metabolism-associated enzymes than fruit stored at 0 and 5 °C, leading to high levels of adenosine triphosphate and energy in fruit. These results indicated that NFT storage can effectively enhance chilling tolerance of nectarine fruit by inducing the metabolism of soluble carbohydrates and energy. [ABSTRACT FROM AUTHOR]

Highlights • Exogenous sucrose delayed the decrease of sucrose content in strawberry fruit. • Sucrose treatment increased glucose and fructose content by promoting AI activity. • Sucrose treatment accelerated the accumulation of four pelargonidin derivatives. • Four pathways involved in anthocyanin synthesis were activated by sucrose signal. Abstract Sucrose acts as a vital signal that modulates fruit ripening. In current study, 50 mM sucrose was applied in strawberry fruit to investigate the regulation of sucrose in anthocyanin synthesis after harvest. The results showed that sucrose treatment increased the contents of glucose, fructose and sucrose, which were 19.76%, 15.83% and 16.50% higher, respectively, compared with control at the end of storage. The increase of glucose and fructose contents resulted from the activation of acid invertase by sucrose treatment. In addition, sucrose treatment specifically increased four pelargonidin derivatives, pelargonidin 3-glucoside, pelargonidin 3-rutinoside, pelargonidin 3-malonylglucoside and pelargonidin 3-methylmalonyglucoside, during the storage. Further, transcriptional profiles and enzyme activities analysis revealed that the accumulation of pelargonidin derivatives was related to the activation of the pentose phosphate pathway, shikimate pathway, phenylpropanoid pathway, and flavonoid pathway. These results provided new insights into the regulation of sucrose on the accumulation of individual anthocyanins. [ABSTRACT FROM AUTHOR]

Graphical abstract Highlights • Rutin and its derivative affect citrinin production by Monascus in some media. • Rutin was less effective in reducing the yield of citrinin. • Troxerutin and α-glucosylrutin showed a greater reduction of citrinin production. • This method for removing citrinin is simple, stable, safe, reliable, and effective. Abstract The mycotoxin citrinin is often produced during fermentation of Monascus products. We studied the effects of flavonoids on citrinin production by Monascus aurantiacus Li AS3.4384 (MALA) by adding rutin, α-glucosylrutin, or troxerutin to the fermentation medium, in a first-of-its-kind study. Appropriate amounts of rutin, α-glucosylrutin, or troxerutin did not affect normal mycelial growth. Addition of 5.0 g/l of rutin only weakly reduced (29.2%) citrinin production, relative to inhibition by 5 g/l α-glucosylrutin or troxerutin (by 54.7% and 40.6%, respectively). In starch inorganic liquid culture media, addition of 20.0 g/l of troxerutin, followed by fermentation for 12 days, reduced citrinin yield by 75.26%. Addition of 15.0 g/l of troxerutin to low-starch peptone liquid fermentation media reduced citrinin yield by 87.9% after 14 days of fermentation, and addition of 30.0 g/l troxerutin to yeast extract sucrose liquid media for 12 days reduced citrinin yield by 53.7%. [ABSTRACT FROM AUTHOR]

Highlights • Two major anthocyanins of sour cherries were isolated. • Sucrose led to reduction in the stabilities of both anthocyanins. • Cherry stem extract (CSE) showed copigmentation effects on these anthocyanins. • Cyanidin-3-glucosylrutinoside caused more stable copigmentation with copigments in CSE. • Interaction between cyanidin-3-rutinoside and copigments in CSE was faster. Abstract Cyanidin-3- O -glucosylrutinoside and cyanidin-3- O -rutinoside were isolated from sour cherry juice concentrate (SCJC). Sucrose and copigments [gallic acid (GA), cherry stem extract (CSE) and pomegranate rind extract (PRE)] were added to these anthocyanins. Effects of sucrose and copigments on the isolated anthocyanins were studied during storage at 20 °C for 140 days. All copigment sources provided protection against degradation effect of sucrose on the isolated anthocyanins before storage. Although GA increased the stabilities of both isolated anthocyanins, only CSE had copigmentation effect on both isolated anthocyanins. Copigmentation effect on cyanidin-3- O -glucosylrutinoside continued throughout storage. However, this effect on cyanidin-3- O -rutinoside continued for only 56 days of storage. Cyanidin-3- O -glucosylrutinoside containing three sugar moieties was more prone to copigmentation than cyanidine-3-rutinoside containing two sugar moieties. Results showed that the presence of more than one anthocyanin in a media changed stability of copigmentation. We recommend CSE for enhancement in colour density, but GA for enhancement in anthocyanin stability. [ABSTRACT FROM AUTHOR]

Highlights • Varying levels of sugars were used to pre-treat Robusta green beans. • Treatment increased the similarity of Robusta to Arabica. • The optimum level of sugar treatment was Robusta soaked in 15F solution. • For coffee aroma the blending ratio can be increased from 20% to 80% Robusta. • The aroma of treated Robusta coffee was more stable than Arabica. Abstract This study attempted to improve Robusta sensory properties by modifying the beans chemical composition. Building on our previous work, which modified bean pH through acid pre-treatment, a model system was developed where, sugar solutions (glucose, fructose, sucrose) were used to pre-treat Robusta coffee beans with the aim to modify the concentration/availability/location of these aroma precursors. Beans were then dried to equal water activity, subjected to equal roast intensity and ground to comparable particle size distributions. The treatment significantly impacted aroma generation during roasting leading to an altered level of pyrazines, furans, ketones, organic acid and heterocyclic nitrogen-containing compounds (p < 0.05). The optimum treatment was 15 g/100 g fructose. 80% treated Robusta could be blended with Arabica in coffee brew without significant aroma differences being perceived when compared to 100% Arabica brew. Furthermore the aroma of the fructose treated Robusta was more stable than Arabica over six weeks accelerated shelflife storage. [ABSTRACT FROM AUTHOR]

Highlights • Sweetness calculation equation for sucrose established: S suc = 12.36 ∗ log 10 C suc + 5.47. • Sweetness index (SI) for five sugars established. • Sweetness calculation equation for four sugars established: S X-ose = 12.36* log 10 (C X-ose SI) +5.47. • Conversion method and equation are more reliable than those in previous studies. Abstract This study's aim is to establish a new sensory sweetness definition and conversion method for five sugars. A "closed-type" question based on triangle test and paired comparison was used for sensory evaluation. The absolute threshold and nine sensory difference threshold values were determined and used to generate a sweet sensory difference strength curve. Defining absolute threshold of sucrose sweetness as 1, the sucrose sweetness at any concentration could be calculated via the curve. After taking the logarithm of each curve, sweetness index was calculated as 1, 1.12, 0.94, 1.29, and 1.25 for sucrose, glucose, fructose, lactose and maltose, respectively. Based on this, each sugar concentration and sweetness could be converted and calculated. Single sugar and mixed-sugars sweetness comparison experiments verified the new sweetness index and sweetness values were more accurate (83.3–100%) than those reported in previous studies. Therefore, this new definition and conversion method established more reliable references for sweet taste sensory applications. [ABSTRACT FROM AUTHOR]

Highlights • The co-melting behavior of sucrose-fructose-glucose mixtures were shown by DSC and microscopy. • Melting temperature of sucrose decreases slightly in glucose but significantly in fructose melts. • Decrease of sucrose melting enthalpy proves co-melting of sugars at lower temperature. Abstract The co-melting behaviour of sugar mixtures, comprising sucrose, glucose and fructose at various binary (3:1, 1:1, 1:3, 1:7, 1:15) and ternary (4:1:1, 2:1:1, 1:1:1, 2:5:5, 2:11:11, 1:4:1, 1:10:1) ratios, was studied using DSC. The melting temperature of sucrose was found to decrease in the presence of either fructose or glucose. In the sugar mixtures, the melting enthalpy of sucrose decreased compared with the proportional calculated values, using the percentage equation, whilst the melting enthalpy of fructose or glucose increased at the same time, implying the melting or dissolution of sucrose together with fructose or glucose. The tested enthalpy is usually higher than the calculated enthalpy for fructose, but lower than the calculated enthalpy for sucrose. Microscopic pictures showed that sucrose crystals could melt and dissolve gradually in the fructose melt liquid. This study provides useful information for both fundamental understanding of sugars co-melting and food industry applications. [ABSTRACT FROM AUTHOR]

Highlights • Chlorination impacts free and bound wheat flour lipids. • It improves the cake batter solvent phase holding capacity of wheat flour. • It improves interaction between the flour components and the batter solvent phase. • Starch and protein networks in chlorinated flour-sucrose gels are well organized. Abstract Chlorine treatment increases the surface hydrophobicity of starch granules of soft wheat flour and reduces its gluten network forming capacity and apparent content of the flour lipids, the latter presumably by forming chlorinated derivatives. It also increases its solvent holding capacity as shown by proton nuclear magnetic resonance (1H NMR) experiments in presence of sucrose. The exchanging protons in the extra-granular space are present in an environment with restricted mobility, indicating strong interactions between chlorinated soft wheat flour (CSWF) components and solvent. Chlorination reduced the capability of free flour lipids to shield starch granules during rapid visco analysis (RVA). The higher viscosity reached in RVA with CSWF was attributed to not only its lipids having different properties but also its protein, resulting in improved starch swelling and amylose leaching. Finally, 1H NMR indicated that starch and protein networks in CSWF-sucrose gels were better structured than in gels prepared from non-chlorinated flour. [ABSTRACT FROM AUTHOR]

Highlights • Glucosylation of stevioside by glucansucrase Gtf180-ΔN-Q1140E yields products with a significantly reduced bitterness. • Structural analysis revealed that stevioside was mainly (α1 → 6)-glucosylated at the steviol C-19 glucosyl moiety. • Optimization of reaction conditions resulted in an efficient conversion and a high product yield. Abstract The adverse health effects of sucrose overconsumption, typical for diets in developed countries, necessitate use of low-calorie sweeteners. Following approval by the European Commission (2011), steviol glycosides are increasingly used as high-intensity sweeteners in food. Stevioside is the most prevalent steviol glycoside in Stevia rebaudiana plant leaves, but it has found limited applications in food products due to its lingering bitterness. Enzymatic glucosylation is a strategy to reduce stevioside bitterness, but reported glucosylation reactions suffer from low productivities. Here we present the optimized and efficient α-glucosylation of stevioside using the mutant glucansucrase Gtf180-ΔN-Q1140E and sucrose as donor substrate. Structures of novel products were elucidated by NMR spectroscopy, mass spectrometry and methylation analysis; stevioside was mainly glucosylated at the steviol C-19 glucosyl moiety. Sensory analysis of the α-glucosylated stevioside products by a trained panel revealed a significant reduction in bitterness compared to stevioside, resulting in significant improvement of edulcorant/organoleptic properties. [ABSTRACT FROM AUTHOR]

Highlights • Sweeteners can hinder consumer acceptance due to bitter aftertaste and lingering. • Adsorption-desorption properties of six sweeteners were evaluated by HPLC. • HFCS had the highest adsorption in the oral cavity, followed by rebaudioside A. • No macro-complexes were formed between sweeteners and salivary proteins. Abstract Adsorption-desorption properties of different sweeteners in the oral cavity were evaluated using high performance liquid chromatography-based methodology. Three low calorie artificial sweeteners (aspartame, acesulfame potassium and sucralose), one steviol glycoside (rebaudioside A), and high fructose corn syrup (HFCS) were examined and compared with sucrose at pH 3 and 7 in a model beverage matrix. Results indicated that HFCS had the highest adsorption in the oral cavity, followed by rebaudioside A and the artificial sweeteners. The physicochemical interaction between sweeteners and salivary proteins did not affect the adsorption properties significantly as validated from a series of characterization techniques. [ABSTRACT FROM AUTHOR]

Highlights • The sol-gel phase transition temperatures range from 32 °C to 44 °C was observed. • The cooling rate significantly contributes the conditions of the gelation phenomenon. • Structural properties of gel depend on the preparation time of sample. • The saccharose negatively effects on the sol-gel phase transition. • The saccharose negatively effect on the structure crosslinking and gel stiffness. Abstract In this paper, starch pastes in the form of solutions and gels were investigated to determine viscoelastic properties and sol-gel phase transition temperatures using rheological methods. The gelatinization process was carried out at a temperature of 95 °C with the use of a pressureless starch cell with a stirrer. Starch pastes obtained were used to determine rheological properties under isothermal conditions (in the temperature range of 45–25 °C) by a cone-plate measurement system. The viscoelastic behavior of the tested medium was confirmed. The range of gelation temperatures was determined and the influence of several factors was discussed, e.g. the effect of sucrose addition and cooling rate on the phenomenon of sol-gel phase transition. In addition, mechanical properties of the obtained starch gel structures were determined using a fractional rheological model. [ABSTRACT FROM AUTHOR]

[Display omitted] • 72 Cassava landraces root metabolomes established with HPLC & GC–MS. • Tryptophan present in all landraces with max. of 2,242.09 µg/100 g DM. • Neighbour joining & PCA analyses revealed four distinct chemotypes. • Scopoletin, DMC, sugars analyzed in 174 acc. and compared with PPD at different ages. • PPD is positively correlated with DMC and negatively correlated with scopoletin. Cassava landraces are impacted by post-harvest physiological deterioration (PPD). 34 primary/secondary metabolites (carotenes, flavonols, indols, phenolic, hydroxycinnamic, and organic acids) were analysed using HPLC/GC–MS in 72 landraces harvested 8 months after planting (MAP) to clarify whether these compounds may play a role in PPD tolerance. Cluster analysis differentiated a first group with high organic acids contents, citric acid being dominant, a second group with landraces high in tryptophan, a third group including landraces with high phenolic and hydroxycinnamic acids content, and a fourth group characterised by 8 carotenoids. PPD tolerant and susceptible landraces were present in each group. To determine if PPD is related to age of harvest, 174 landraces were harvested at 6, 8, 10 and 12 MAP. Scopoletin, sucrose and glucose were analysed. PPD was positively correlated with DMC and negatively correlated with scopoletin at all ages of harvest. Scopoletin is a useful biomarker to characterize landraces. [ABSTRACT FROM AUTHOR]

• Stable granular packing could resist the influence of NaCl/sucrose. • Amylopectin structure is critical to thermal properties as affected by co-solute. • Co-solute-induced change in retrogradation varied with amylose molecular structure. • Effect of NaCl on gel viscoelasticity might reflect amylopectin internal structure. The influences of sodium chloride (NaCl)/sucrose on starch properties as affected by starch structural characteristics are little understood. In this study, the effects were observed in relation to the chain length distribution (from size exclusion chromatography) and granular packing (inferred through morphological observation and determination of swelling factor and paste transmittance) of starches. Adding NaCl/sucrose dramatically delayed the gelatinization of starch that had a high ratio of short-to-long amylopectin chains and had loose granular packing. The effects of NaCl on the viscoelasticity of gelatinizing starch were related to the flexibility of amylopectin internal structure. Effects of NaCl/sucrose on starch retrogradation varied with starch structure, co-solute concentration, and analytical method. The co-solute-induced changes in retrogradation were highly associated with amylose chain length distribution. Sucrose strengthened the weak network formed by short amylose chains, while the effect was not significant on amylose chains that were capable of forming strong networks. [ABSTRACT FROM AUTHOR]

[Display omitted] • First workflow for thyme authenticity by non-destructive 1H NMR fingerprinting. • Moroccan, Polish, and Spanish thyme were distinguished by supervised OPLS-DA. • Multivariate statistics also discriminated between untreated and sterilized thyme. • Eight major metabolites were identified as markers using untargeted metabolomics. • First 1H NMR, GC–HRMS, and LC–HRMS mid-level data fusion study performed in thyme. Thyme is a culinary herb highly susceptible to increasing mislabeling occurring in the spice industry. In this study, proton nuclear magnetic resonance spectroscopy (1H NMR) combined with multivariate statistics was successfully applied with two authenticity purposes: (1) tracing thyme metabolic differences among three relevant geographical regions (Morocco, Spain, and Poland), and (2) assessing the influence of sterilization processing on the metabolic fingerprint. Multivariate data analysis provided six and seven key geographical and processing markers, respectively, including thymol, organic acids, chlorogenic acid, and some carbohydrates (e.g., sucrose). Additionally, for the first time, a mid-level data fusion approach was tested for thyme authenticity combining three complementary and synergic analytical platforms: gas and liquid chromatography coupled with high-resolution mass spectrometry, and 1H NMR spectroscopy, providing a comprehensive metabolomics insight into the origin and processing effects on thyme fingerprinting, and opening the path to new metabolomics approaches for quality control in the spice industry. [ABSTRACT FROM AUTHOR]

• The influence of sucrose particle size, and sucrose source on the volatile and aroma of sponge cakes was investigated. • Sponge cakes were formulated from cane or beet sucrose at three sucrose particle sizes (small, control and large). • Particle size had the greatest impact on overall volatile abundance and aroma properties. • Small particle size formulas yielded higher Maillard reaction compounds (pyrazines). The influence of sucrose source and particle size was investigated in relation to the volatile and aromatic properties of sponge cakes. Six sponge cake formulations were studied using two sucrose sources (sugarbeet and sugarcane), at two particle sizes (large and small) with controls. Volatiles profiles and odour active compounds were identified by gas chromatography mass spectrometry and olfactometry. Sixty two volatile compounds were identified, incorporating twenty five odour active compounds/co-eluting compounds, with 5 odours perceived without any corresponding volatile. Particle size had the greatest impact on volatile abundance, with particle size especially influencing pyrazine abundance. Five odour active volatiles (methional, furfural, 2,3-dimethylpyrazine, heptanal and (E)-2-octenal) contributed most to the aroma of these sponge cakes. Small particle size particularly from sugarbeet yielded higher levels of some Maillard and caramelisation reaction compounds, such as furfural (spicy/ bready), where larger particle size supressed volatile abundance in comparison to the control. [ABSTRACT FROM AUTHOR]

This study was intended to evaluate the quality parameters of 58 Apis mellifera honey samples, from different regions in the Sultanate of Oman. Physicochemical analyses were carried out and examined according to the Gulf Standardization Organization (GSO). The results revealed that 64.4% of the samples were failing to meet the GSO standards due to acidity, hydroxyl methyl furfural (HMF), diastase, sucrose, and glucose & fructose. Acidity and HMF were above the limits in 30% and 29% of the failed samples respectively, where diastase and total glucose & fructose were below the limits in 25% and 5% respectively. Sucrose was above the limits in 11% of the failed samples. The unconformity of the analyzed honey samples to GSO standards could be due to stage of harvesting, process and storage conditions. Therefore, it’s important to reconsider the whole process of honey production in Oman in order to improve the technology and honey quality. [ABSTRACT FROM AUTHOR]

Sucrose and its constituent components, glucose and fructose, are major determinants of sweetness in fruits and cooking quality in plant storage organs, such as potato tubers. Accurate monitoring of these sugars provides a predictive assessment of plant product quality. High Performance Liquid Chromatography (HPLC) coupled to a variety of detectors has been the method of choice for selective detection and quantitation of sugars in plant tissues. However, sugar extraction can be time-consuming to obtain a clean and concentrated sample suitable for accurate quantitation. Herein, we took advantage of a modern HPLC system coupled to a highly sensitive high-resolution high-accuracy mass spectrometer to develop a rapid method for measuring glucose, fructose, and sucrose in plant tissues. The method was validated in mature potato tubers and strawberry fruits and it proved to be highly accurate and robust with minimal sample care requirements. [ABSTRACT FROM AUTHOR]

Steviol glycosides from Stevia rebaudiana leaves are used in stevia-based sweeteners for their intense sweetness and low calories. Rebaudioside D is present in leaves in minute quantities (∼0.4–0.5% w/w total dry weight), but it is ∼350 times sweeter than sucrose, and sweeter than the more abundant rebaudioside A and stevioside. In the present study, pathways for rebaudioside D synthesis and UDP-glucose recycling were developed by coupling recombinant UDP-glucosyltransferase UGTSL2 from Solanum lycopersicum and sucrose synthase StSUS1 from Solanum tuberosum . Reaction parameters, including substrate ratio, sucrose concentration, temperature, crude extract concentration, and reaction time, were evaluated, and 17.4 g/l of rebaudioside D (yield = 74.6%) was obtained from 20 g/l of rebaudioside A after 20 h, using UDP or UDP-glucose in recombinant cell crude extracts. Extending the reaction time generated rebaudioside M2 from further glycosylation of rebaudioside D. K m values for UGTSL2 indicated a higher affinity for rebaudioside D than for rebaudioside A. [ABSTRACT FROM AUTHOR]

Dramatic increase in NaCl consumption lead to sodium intake beyond health guidelines. KCl substitution helps reduce sodium intake but results in a bitter-metallic off-taste. Two disaccharides, trehalose and sucrose, were tested in order to untangle the chemical (increase in effective concentration of KCl due to sugar addition) from the sensory effects. The bitter-metallic taste of KCl was reduced by these sugars, while saltiness was enhanced or unaltered. The perceived sweetness of sugar, regardless of its type and concentration, was an important factor in KCl taste modulation. Though KCl was previously shown to increase the chemical activity of trehalose but not of sucrose, we found that it suppressed the perceived sweetness of both sugars. Therefore, sensory integration was the dominant factor in the tested KCl-sugar combinations. [ABSTRACT FROM AUTHOR]

In the fruit juice industry, high pressure (HP) processing has become a commercial success. However, enzymatic browning, cloud loss, and flavor changes during storage remain challenges. The aim of this study is to combine kiwifruit puree and HP pasteurization (600 MPa/3 min) to stabilize cloudy apple juice during storage at 4 °C. A wide range of targeted and untargeted quality characteristics was evaluated using a multivariate approach. Due to high ascorbic acid content and high viscosity, kiwifruit puree allowed to prevent enzymatic browning and phase separation of an apple-kiwifruit mixed juice. Besides, no clear changes in organic acids, viscosity, and particle size distribution were detected in mixed juice during storage. Sucrose of apple and mixed juices decreased with glucose and fructose increasing during storage. The volatile changes of both juices behaved similar, mainly esters being degraded. Sensory evaluation demonstrated consumer preferred the aroma of mixed juice compared to apple juice. [ABSTRACT FROM AUTHOR]

Amperometric biosensor utilizing FAD-dependent glucose dehydrogenase (FAD-GDH) for a specific sucrose monitoring in green coffee is described. FAD-GDH was co-immobilized with invertase and mutarotase on a thin-layer gold planar electrode using chitosan. The biosensor showed a wide linearity (from 10 to 1200 μM), low detection limit (8.4 μM), fast response time (50 s), and appeared to be O2 independent. In addition the biosensors exhibited a good operational (3 days) and storage (1 year) stability. Finally, the results achieved from the biosensor measurements of sucrose in 17 samples of green coffee (Coffea arabica, C. canephora and C. liberica) were compared with those obtained by the standard HPLC method. The good correlation among results of real samples, satisfactory analytical performance and simple use of the presented biosensor make it suitable for application in coffee industry. [ABSTRACT FROM AUTHOR]

Quantitative structure activity relationship (QSAR) models appear to be an ideal tool for quick screening of promising candidates from a vast library of molecules, which can then be further designed, synthesized and tested using a combination of rigorous first principle simulations, such as molecular docking, molecular dynamics simulation and experiments. In this study, QSAR models have been built with an extensive dataset of 487 compounds to predict the sweetness potency relative to sucrose (ranging 0.2–220,000). The whole dataset was randomly split into training and test sets in a 70:30 ratio. The models were developed using Genetic Function Approximation (R test 2  = 0.832) and Artificial Neural Network (R test 2  = 0.831). Our models thus offer a convenient route for fast screening of molecules prior to synthesis and testing. Additionally, this study can supplement a molecular modelling approach to improve binding of molecules with sweet taste receptors, leading to design of novel sweeteners. [ABSTRACT FROM AUTHOR]

The radiolytic decomposition of glucose, fructose, sucrose, ascorbic acid (H 2 A) and dehydroascorbic acid (DHAA), induced by ionizing radiation, was investigated. Aqueous solutions of these compounds were gamma-irradiated at doses of 0.5, 0.7 and 1 kGy, with a dose rate of 0.04 kGy/min, in the presence of air, followed by HPLC, HPLC-MS, EPR, and UV–VIS spectroscopy analysis of the resulting mixtures. Carboxylic acids, 2-furaldehyde, 5-hydroxymethyl-2-furaldehyde, furfuryl alcohol, 2-furoic acid and 2( 5H )-furanone were identified in solutions of sugars and vitamin C, by comparing with standards. EPR studies exhibited a higher amount of free radicals for fructose than the other sugars, and more for DHAA than H 2 A. Additionally, spectroscopic determinations showed a typical UV–VIS absorption for furans during the storage of irradiated solutions. Formation and decomposition routes for furanoids and aliphatic carboxylic acids, and mechanistic pathways for the radiolytic induced rupture of glucose, fructose, sucrose and vitamin C have been proposed. [ABSTRACT FROM AUTHOR]

In this study, four jam formulations were developed, starting with the basic formulation (Jam 1) containing sucrose and without added pectin. Sucrose was partially (50%) replaced by stevioside in formulations of Jam 2 and 3, while in Jam 4 sucrose was completely replaced by fructose and stevioside, making this formulation suitable for diabetic patients. Jam formulations 1 and 2, prepared without added pectin, were thermally stable in the temperature range of 25–90 °C, which indicate their potential use as fruit fillings. Jam formulations 3 and 4 were assessed by the sensory panel as more spreadable since tomato pomace particles are incorporated in pectin network which acts as a lubricant. Jam formulations were characterized by a lower total carbohydrate content (17.23–43.81%) and lower energy value (87.1–193.7 kcal/100 g) when compared to commercial products. Tomato pomace jams contained 15–20 times more dietary fibre than commercial apricot jam. [ABSTRACT FROM AUTHOR]

• Batch-wise is compared to continuous production in cookies manufacturing. • HSI-NIR reveals surface compositional inhomogeneity with the manufacturing procedure. • HSI-NIR and chemometrics are a perfect combination to study food production. • PCA, ASCA and HSI-NIR are essential to understand physicochemical effects in food. The Danish buttered cookie is a famous confectionery product. Its success makes manufacturing of the large volumes required challenging, introducing the need for different strategies to increase production while maintaining a high-quality standard. Two manufacturing lines used are batch-wise and continuous dough mixing. Despite the recipe being the same, the outcome of the two production types differs in texture and external appearance. While this does not infringe on the quality, changes in texture are observable. This manuscript analyses the physicochemical differences of the cookies after baking using Near Infrared hyperspectral imaging and Chemometrics. The study demonstrates that the changes in texture between batch and continuous production are mostly due to the difference in crystalline sucrose emerging in invisible spots on or near the surface of the cookies and a higher tendency of migrated butter-fat spots on the surface of the cookies for the continuous manufacturing procedure. [ABSTRACT FROM AUTHOR]

[Display omitted] • Sucrose ester can alleviate the agglomeration behavior of BSDF via high-pressure homogenization. • Particle size decreased and ζ-Potential of BSDF increased due to the surface modification of SE. • Shear stress and viscoelasticity of BSDF solution decreased with the addition of SE. • Relative crystallinity and thermal stability of BSDF were minimized when SE was added at 5 g/L. • Mechanism of electrostatic repulsion effectively alleviates the aggregation of BSDF. High-pressure homogenization (HPH) is a physical modification method that can rapidly reduce the particle size of bamboo shoot dietary fiber (BSDF), but it can lead to agglomeration. Therefore, the effects of the addition of sucrose ester (SE) to alleviate the agglomeration of BSDF during HPH were investigated. Compared with BSDF without added SE, BSDF obtained the smallest particle size (276.5 nm) and highest ζ-Potential (53.6 mV) when SE was 5 g/L. Water-holding capacity, oil-holding capacity, swelling capacity, and b* increased, whereas L* and a* decreased significantly with the addition of SE. The shear stress and viscoelasticity of BSDF solution were minimized when 5 g/L SE was added. SE reduced relative crystallinity and thermal stability of BSDF. SE could effectively alleviate the aggregation of BSDF through the mechanism of electrostatic repulsion. This study highlights an innovative and promising strategy for alleviating the agglomeration behavior of BSDF during HPH treatment. [ABSTRACT FROM AUTHOR]

• Ascorbic acid (AsA) treatment inhibited StGST but enhanced StPHO1 and StLOX5 gene expression. • AsA-treated samples had higher levels of starch content and starch metabolism. • AsA treatment increased amylase and lipoxygenase activity. • AsA treatment inhibited sucrose degradation and fatty acid synthesis. Ascorbic acid (AsA) inhibits wound healing in fresh-cut potatoes (FCP); however, the comprehensive regulatory mechanisms of the chemical during wound healing remain unclear. Here, physiobiochemical, transcriptomic, and metabolomic analyses were performed. In total, 685 differentially expressed genes (DEGs) and 1921 differentially accumulated metabolites (DAMs) were identified between control and AsA-treated samples. The level of the majority of DEGs expression and DAMs abundance in AsA-treated samples were similar to data of newly cut samples. The collective data indicated that the AsA treatment inhibited wound healing in FCPs by regulating glutathione metabolism, enhancing starch metabolism, and inhibiting phenylalanine metabolism, sucrose degradation, and fatty acid synthesis. Major genes and metabolites affected by AsA treatment included StGST , StPAL , StPHO1 and StLOX5 , and starch, sucrose, and linoleic acid. AsA treatment increased starch content and amylase and lipoxygenase activity and decreased free fatty acid level. Our research provides fundamental insights into wound healing mechanisms in FCP. [ABSTRACT FROM AUTHOR]