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Process biofilms of Bacillus licheniformis present worldwide problem to the dairy industry because of their relevance to food spoilage and quality issues. In this study, metabolites of Lactobacillus plantarum were found to effectively inhibit biofilms formed by B. licheniformis. The biofilm formation was delayed by 10 h by the addition of L. plantarum cell free supernatant and controlling pH of the medium. Confocal laser scanning microscope images showed that L. plantarum metabolites decreased the number of B. licheniformis cells adhered to stainless steel and glass surfaces. Gel filtration chromatography results showed that B. licheniformis utilized the peptides in the growth medium (tryptic soy broth) with the molecular weights ranging from 613 to 1486 Da, and produced plentiful small peptides with the molecular weights ranging from 181 to 613 Da. Moreover, 3–10 kDa components of L. plantarum ultra-filtrates were proved to significantly inhibit biofilm formation by B. licheniformis. Thus, the metabolites produced by L. plantarum provide a novel approach for the prevention of B. licheniformis biofilms in the dairy industry. • Lactobacillus plantarum supernatant effectively inhibited the biofilm formation by Bacillus licheniformis. • L. plantarum inhibited planktonic cell growth of B. licheniformis in co-cultivation. • B. licheniformis preferred an alkaline environment for planktonic growth, and a neutral environment for biofilm formation. • B. licheniformis utilized and decomposed the peptides into smaller ones in tryptic soy broth. • 3–10 kDa components of L. plantarum metabolites obviously weakened biofilm formation of B. licheniformis. [ABSTRACT FROM AUTHOR]

Biofilms on the surface of dairy processing equipment act as potential reservoirs of microbial contamination, which may affect product quality and cause substantial risks for consumer health. Biofilm-forming capacity of a high number of psychrotrophic bacterial species of dairy origin at low temperatures has been poorly reported. This work aimed to explore the biofilm-forming potential of bacterial strains (previously identified from Chinese raw milk) belonging to 72 RAPD based genetic groups (representing 11 predominant genera and 61 species) at 7 °C. Results showed that biofilm formation is a strain-dependent trait, and 25, 10, 19 RAPD groups were weak, moderate and high biofilm formers, respectively, based on results of the crystal violet staining. Isolates of Pseudomonas showed the high tendency to form biofilms. The cell numbers recovered on tryptic soy broth agar plates from surface biofilms ranged from 1.15 to 6.63 log CFU/cm 2 , and the maximum number was detected for Pseudomonas azotoformans (a) (6.63 log CFU/cm 2 ) followed by Serratia quinivorans (6.39 log CFU/cm 2 ) and Pseudomonas libanensis (6.34 log CFU/cm 2 ). In the presence of skim milk, cell numbers decreased to a range from 1.04 to 6.1 log CFU/cm 2 , and high cell number was observed in case of P. azotoformans (a) (6.1 log CFU/cm 2 ), P. libanensis (6.07 log CFU/cm 2 ) and S. quinivorans (5.84 log CFU/cm 2 ). A marked variation in biofilm-forming capacity was observed within the strains belonging to the same species. A moderate correlation (r = 0.42) between the crystal violet assay of polystyrene and methods based on cell enumeration on coupons was observed. Meanwhile, the cell number of Aeromonas hydrophila recovered from stainless steel increased in co-culture with Pseudomonas fluorescens (a). The results expand the knowledge of biofilm-forming capacity of psychrotrophic bacteria of dairy origin. [ABSTRACT FROM AUTHOR]