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Start Over Author "Li, Shunfeng" Journal food science and biotechnology
4 results on '"Li, Shunfeng"'

3. Extraction of polysaccharides under vacuum condition from Lentinus edodes stipe and their antioxidant activities in vitro

Author

Wang Anjian, Liu Lina, Xu Fangfang, Tian Guangrui, and Li Shunfeng

Subjects
0106 biological sciences, chemistry.chemical_classification, Chromatography, biology, Chemistry, Extraction (chemistry), 04 agricultural and veterinary sciences, biology.organism_classification, Polysaccharide, 040401 food science, 01 natural sciences, Applied Microbiology and Biotechnology, Cell wall, chemistry.chemical_compound, 0404 agricultural biotechnology, Stipe (botany), 010608 biotechnology, medicine, Lentinus, Response surface methodology, Hydrogen peroxide, Food Science, Biotechnology, Activated carbon, medicine.drug
Abstract

To improve the efficiency of polysaccharide extraction, vacuum technology was used to breakdown the cell wall to extract polysaccharides from Lentinus edodes stipe, and the optimal parameters were optimized by response surface methodology. Then the antioxidant activities in vitro of the polysaccharides were evaluated. Results showed that the optimal conditions for polysaccharide extraction were temperature of 62 °C, vacuum of 0.08 MPa, stirring speed of 1200 r/min, time of 25 min and material/liquid ratio of 1:26 (g/mL). Under these conditions, the yield of polysaccharide was 4.28%. The decolorization rate and deproteinization rate of polysaccharides treated by kaolin and hydrogen peroxide were higher than that of activated carbon. However, there was no significant influence on the polysaccharide retention rate, that was about 80%. Results also indicated that polysaccharide treated with activated carbon could obtain higher antioxidant activity.

Published
2018

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