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Vibrio parahaemolyticus is a major cause of foodborne gastroenteritis in China, Japan, and other countries. The pandemic O3:K6 clone, which harbors thermostable direct hemolysin [tdh] gene and toxRS/new gene, is mainly responsible for the foodborne outbreaks after 1995. Previous studies showed that genes in the pathogenicity island-1 (VPaI-1) and VPaI-5 are harbored only by pandemic strains, whereas genes in VPaI-7 and type III secretion system 2 are closely associated with tdh-positive strains of V. parahaemolyticus. In this study, we examined the distribution of genes encoding VPaI-2, VPaI-3, VPaI-4, VPaI-6, type VI secretion systems (T6SS), biofilm, and type I pilus in 71 food and 116 clinical strains of V. parahaemolyticus. The results showed that most of the pandemic strains of V. parahaemolyticus harbored the complete genes of VPaI-2, T6SS, and type I pilus. In contrast, most of the pathogenic strains (harboring tdh gene or TDH-related hemolysin [trh] gene) and nonpathogenic strains (harboring neither tdh gene nor trh gene) contained partial genes of VPaI-2, T6SS, and type I pilus. Genes of VPaI-4 were exclusively present in the pandemic strains. Genes of VPaI-3 were present in most of the pandemic strains and a small percentage of nonpathogenic strains, mainly O3:K6 strains. VPaI-6 and biofilm-associated genes were harbored by almost all the strains, irrespective of their pandemic, pathogenic, or nonpathogenic traits.

Vibrio parahaemolyticus is a major foodborne pathogen in China, Japan, and other Asian countries. In this study, we collected 437 strains of V. parahaemolyticus and investigated their serotypes, distribution of virulence genes, and presence of pandemic O3:K6 clone strains. A total of 327 strains were isolated from food and 110 strains were isolated from active surveillance hospitals or food outbreaks during 2005 to 2008. Presence of the tdh and trh genes is the key characteristic of virulent strains. Positive for both the tdh gene and group-specific polymerase chain reaction is the key characteristic of pandemic strains. A total of 9 O serogroups and 62 serovars were identified in all strains. Nine O serogroups and 56 serovars existed in 327 foodborne strains, and 6 O serogroups and 20 serovars existed in 110 clinical strains. Among the 327 food isolates, 6 isolates belonged to the pandemic clone with the orf8 gene (1 isolate was O1:KUT (untyped) and 5 isolates were O3:K6) and 4 isolates carried the trh gene (2 isolates belonged to O1:KUT and 2 isolates belonged to O5:KUT and O5:K17). Seventy-nine percent of the clinical isolates were pandemic strains, 9.4% of which lacked the orf8 gene. O3:K6 was the main serovar of the pandemic strains accounting for 83.5% of the clinical pandemic strains. Pandemic clonal serovars included O3:K6, O1:KUT, O1:K25, O1:K26, and O4:K68, and the newly emerging serovars O1:K36, O3:K25, and O3:K68 identified in the current study. O3:K6 was the dominant serovar in pandemic strains. All pandemic isolates had identical arbitrarily primed polymerase chain reaction fragment patterns, but did not share similar antibiotic sensitivity patterns. These results suggest that high serodiversity of V. parahaemolyticus was present in foodborne strains. Pathogenic isolates, especially pandemic isolates, were present in high-priced iced seafood and became the potential risk factor in food.

Vibrio parahaemolyticus is one of the most important pathogens capable of causing foodborne gastroenteritis in China, Japan, and other countries. Pathogenic V. parahaemolyticus has been known to produce either thermostable direct hemolysin (TDH), TDH-related hemolysin (TRH), or both. The emergence of a new clone in 1995, V. parahaemolyticus O3:K6, has resulted in the first documented pandemic spread of V. parahaemolyticus. In this study, 235 isolates from clinical and food sources were characterized by determining the presence of known virulence factors (tdh, trh), systematic genetic markers (toxRS/new, pandemic group-specific sequence [PGS], orf8) specific for V. parahaemolyticus O3:K6 clone and its clonal derivatives, three important genomic islands (GIs) (VPaI-1, VPaI-5, and VPaI-7), and two type III secretion systems (T3SS1 and T3SS2). Our results showed that all 235 isolates harbored all or part of the T3SS1 genes. All the 103 tdh-positive strains harbored all or part of the VPaI-7 and T3SS2 genes. A total of 91 isolates including six foodborne isolates belonged to a pandemic clone in which eight isolates lacked orf8. All pandemic strains harbored VPaI-1 and VPaI-5 except one O4:K68 strain that lacked VPaI-5 altogether. Twelve clinical pathogenic strains had VPaI-7 and T3SS2 but lacked VPaI-1 and VPaI-5. Thirteen nonpathogenic clinical strains and 119 foodborne strains, including six foodborne pathogenic trh-positive strains, only harbored T3SS1 genes. These results indicated that O3:K6 and its serovariants were the main pandemic clone in China. VPaI-1 and VPaI-5 genes were specifically correlated with pandemic strains while VPaI-7 and T3SS2 were closely associated with tdh-positive strains.