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Structural complexity of trees is related to various ecological processes and ecosystem services. To support management for complexity, there is a need to assess the level of structural complexity objectively. The fractal-based box dimension (Db) provides a holistic measure of the structural complexity of individual trees. This study aimed to compare the structural complexity of Scots pine (Pinus sylvestris L.) trees assessed with Db that was generated with point cloud data from terrestrial laser scanning (TLS) and aerial imagery acquired with an unmanned aerial vehicle (UAV). UAV imagery was converted into point clouds with structure from motion (SfM) and dense matching techniques. TLS and UAV measured Db-values were found to differ from each other significantly (TLS: 1.51 ± 0.11, UAV: 1.59 ± 0.15). UAV measured Db-values were 5% higher, and the range was wider (TLS: 0.81–1.81, UAV: 0.23–1.88). The divergence between TLS and UAV measurements was found to be explained by the differences in the number and distribution of the points and the differences in the estimated tree heights and number of boxes in the Db-method. The average point density was 15 times higher with TLS than with UAV (TLS: 494,000, UAV 32,000 points/tree), and TLS received more points below the midpoint of tree heights (65% below, 35% above), while UAV did the opposite (22% below, 78% above). Compared to the field measurements, UAV underestimated tree heights more than TLS (TLS: 34 cm, UAV: 54 cm), resulting in more boxes of Db-method being needed (4–64%, depending on the box size). Forest structure (two thinning intensities, three thinning types, and a control group) significantly affected the variation of both TLS and UAV measured Db-values. Still, the divergence between the two approaches remained in all treatments. However, TLS and UAV measured Db-values were consistent, and the correlation between them was 75%. [ABSTRACT FROM AUTHOR]