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In this paper, a generalized fractional three-species food chain model with delay is investigated. First, the existence of a positive equilibrium is discussed, and the sufficient conditions for global asymptotic stability are given. Second, through selecting the delay as the bifurcation parameter, we obtain the sufficient condition for this non-control system to generate Hopf bifurcation. Then, a nonlinear delayed feedback controller is skillfully applied to govern the system's Hopf bifurcation. The results indicate that adjusting the control intensity or the control target's age can effectively govern the bifurcation dynamics behavior of this system. Last, through application examples and numerical simulations, we confirm the validity and feasibility of the theoretical results, and find that the control strategy is also applicable to eco-epidemiological systems. [ABSTRACT FROM AUTHOR]