1. Elevated cardiac hemoglobin expression is associated with a pro-oxidative and inflammatory environment in primary mitral regurgitation.
- Author
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Manzoor, Shajer, Kane, Mariame Selma, Grenett, Maximiliano, Oh, Joo-Yeun, Pat, Betty, Lewis, Clifton, Davies, James E., Steele, Chad, Patel, Rakesh P., and Dell'Italia, Louis J.
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MITRAL valve insufficiency , *GENE expression , *INTERSTITIAL cells , *IN situ hybridization , *HEMOGLOBINS , *HAPTOGLOBINS , *GLOBIN genes - Abstract
Primary mitral regurgitation (PMR) is associated with oxidative and inflammatory myocardial damage. We reported greater exosome hemoglobin (Hb) in pericardial fluid (PCF) versus plasma, suggesting a cardiac source of Hb. Test the hypothesis that Hb is produced in the PMR heart and is associated with increased inflammation. Hb gene expression for subunits alpha (HBA) and beta (HBB) was assessed in right atria (RA), left atria (LA) and left ventricular (LV) tissue from donor hearts (n = 10) and PMR patient biopsies at surgery (n = 11). PMR patients (n = 22) had PCF and blood collected for macrophage markers, pro-inflammatory cytokines, and matrix metalloproteinases (MMPs). In-situ hybridization for HBA mRNA and immunohistochemistry for Hb-alpha (Hbα) and Hb-beta (Hbβ) protein was performed on PMR tissue. HBA and HBB genes are significantly increased (>4-fold) in RA, LA, and LV in PMR vs. normal hearts. In PMR tissue, HBA mRNA is expressed in both LV cardiomyocytes and interstitial cells by in-situ hybridization; however, Hbα and Hbβ protein is only expressed in interstitial cells by immunohistochemistry. PCF oxyHb is significantly increased over plasma along with low ratios (<1.0) of haptoglobin:oxyHb and hemopexin:heme supporting a highly oxidative environment. Macrophage chemotactic protein-1, tumor necrosis factor-α, interleukin-6, and MMPs are significantly higher in PCF vs. plasma. There is increased Hb production in the PMR heart coupled with the inflammatory state of the heart, suggests a myocardial vulnerability of further Hb delivery and/or production during cardiac surgery that could adversely affect LV functional recovery. [Display omitted] • Hemoglobin is produced by cardiomyocytes and interstitial cells in the PMR heart. • Cell-free hemoglobin is elevated and haptoglobin levels are depleted in PCF vs. plasma. • PCF has a greater oxidative environment vs. plasma. • A gradient of pro-inflammatory cytokines, chemoattractants, and MMPs are higher in PCF vs. plasma supporting a myocardial source. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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