1. Hemin-induced Erk1/2 activation and heme oxygenase-1 expression in human umbilical vein endothelial cells.
- Author
-
Chen, Nan, Shao, Wei, Lv, Pingping, Zhang, Shuluo, Chen, Yingying, Zhu, Li, Lu, Yuan, and Shen, Yueliang
- Subjects
- *
HEME oxygenase , *PHOSPHORYLATION , *GENETIC transduction , *BACTERIOPHAGES , *VASCULAR grafts , *CHEMICAL reactions - Abstract
Hemin has been reported to be protective in the pathological process, but its protective mechanisms have not been precisely defined. Hemin could induce Erk1/2 phosphorylation in astrocyte. Erk1/2 phosphorylation has been proved to be involved in many growth signals cellular transduction. However, little study has been conducted as to the relationship between hemin and Erk1/2 activation in human umbilical vein endothelial cells (HUVECs). The present study aimed to investigate the relationship between hemin and Erk1/2 phosphorylation in HUVECs. The results showed that low concentration of hemin induced and sustained phosphorylation of Erk1/2 for a long time. The HO inhibitor protoporphyrin IX zinc (II) abrogated phosphorylation of Erk1/2 induced by hemin. Biliverdin, one of the metabolites of hemin, obviously induced the Erk1/2 phosphorylation in HUVECs. Both hemin and biliverdin promoted HUVEC cell growth. The results strongly suggested that hemin could induce and sustain Erk1/2 phosphorylation in HUVECs by way of HO-1 induction and biliverdin produced from HO-1 catalysing hemin degradation. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF