1. Expression of AQP3 protein in hAECs is regulated by Camp-PKA-CREB signalling pathway.
- Author
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Hua Y, Ding S, Zhang W, Zhou Q, Ye W, Chen M, and Zhu X
- Subjects
- Aquaporin 3 genetics, Cell Proliferation, Cells, Cultured, Colforsin pharmacology, Cyclic AMP metabolism, Cyclic AMP Response Element-Binding Protein metabolism, Cyclic AMP-Dependent Protein Kinases metabolism, Gene Expression Regulation, Humans, Isoquinolines pharmacology, Signal Transduction, Sulfonamides pharmacology, Up-Regulation, Aquaporin 3 metabolism, Cyclic AMP-Dependent Protein Kinases physiology
- Abstract
Previous studies by others and our group have demonstrated the expression of AQP3 protein in human chorioamniotic membranes. Here, we investigated whether cyclic adenosine monophosphate (cAMP) up-regulation of aquaporin 3 (AQP3) protein expression in human amniotic epithelial cells (hAECs) is mediated by protein kinase A (PKA) dependent or independent pathway. Cells were treated with various concentrations of Forskolin, SP-cAMP, H-89 at various time intervals or optimal concentration of Forskolin in combination with H-89 in the blocking experiments. Forskolin significantly increased cAMP levels and the expression of PKA, p-CREB and AQP3. SP-cAMP had similar effects. H-89 inhibited PKA, p-CREB and AQP3 protein expression, and attenuated the stimulatory effect of Forskolin. These results show that the AQP3 protein expression in hAECs was regulated by cAMP-PKA-CREB signal pathway. A relatively short biological half life of AQP3 renders its rapid responsiveness to regulation.
- Published
- 2015
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