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Start Over Author "Ana B. Castro" Journal frontiers in cellular and infection microbiology
2 results on '"Ana B. Castro"'

1. Antimicrobial Mechanisms of Leucocyte- and Platelet Rich Fibrin Exudate Against Planktonic Porphyromonas gingivalis and Within Multi-Species Biofilm: A Pilot Study

Author

Fabio Rodríguez Sánchez, Tim Verspecht, Ana B. Castro, Martine Pauwels, Carlos Rodríguez Andrés, Marc Quirynen, and Wim Teughels

Subjects
biofilms, L-PRF, wound healing, antimicrobial mechanism of action, infection disease, oral microbiota, Microbiology, QR1-502
Abstract

Leucocyte- and platelet rich fibrin (L-PRF) is an autologous biomaterial used in regenerative procedures. It has an antimicrobial activity against P. gingivalis although the mechanism is not fully understood. It was hypothesized that L-PRF exudate releases hydrogen peroxide and antimicrobial peptides that inhibit P. gingivalis growth. Agar plate and planktonic culture experiments showed that the antimicrobial effect of L-PRF exudate against P. gingivalis was supressed by peroxidase or pepsin exposure. In developing multi-species biofilms, the antimicrobial effect of L-PRF exudate was blocked only by peroxidase, increasing P. gingivalis growth with 1.3 log genome equivalents. However, no effect was shown on other bacteria. Pre-formed multi-species biofilm trials showed no antimicrobial effect of L-PRF exudate against P. gingivalis or other species. Our findings showed that L-PRF exudate may release peroxide and peptides, which may be responsible for its antimicrobial effect against P. gingivalis. In addition, L-PRF exudate had an antimicrobial effect against P. gingivalis in an in vitro developing multi-species biofilm.

Published
2021
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2. Antimicrobial Mechanisms of Leucocyte- and Platelet Rich Fibrin Exudate Against Planktonic Porphyromonas gingivalis and Within Multi-Species Biofilm: A Pilot Study

Author

Ana B. Castro, Wim Teughels, Martine Pauwels, Marc Quirynen, Tim Verspecht, Fabio Rodríguez Sánchez, and Carlos Rodríguez Andrés

Subjects
Microbiology (medical), Exudate, Antimicrobial peptides, Immunology, PROGRESSION, wound healing, Pilot Projects, Microbiology, DISEASE, Agar plate, Cellular and Infection Microbiology, Anti-Infective Agents, Platelet-Rich Fibrin, medicine, Porphyromonas gingivalis, Original Research, RISK, Science & Technology, biology, PLASMA, Chemistry, Biofilm, L-PRF, Exudates and Transudates, Antimicrobial, biology.organism_classification, oral microbiota, Plankton, QR1-502, digestive system diseases, Infectious Diseases, infection disease, Biofilms, biology.protein, antimicrobial mechanism of action, GROWTH, medicine.symptom, biofilms, PERIODONTITIS, Life Sciences & Biomedicine, Bacteria, Peroxidase
Abstract

Leucocyte- and platelet rich fibrin (L-PRF) is an autologous biomaterial used in regenerative procedures. It has an antimicrobial activity against P. gingivalis although the mechanism is not fully understood. It was hypothesized that L-PRF exudate releases hydrogen peroxide and antimicrobial peptides that inhibit P. gingivalis growth. Agar plate and planktonic culture experiments showed that the antimicrobial effect of L-PRF exudate against P. gingivalis was supressed by peroxidase or pepsin exposure. In developing multi-species biofilms, the antimicrobial effect of L-PRF exudate was blocked only by peroxidase, increasing P. gingivalis growth with 1.3 log genome equivalents. However, no effect was shown on other bacteria. Pre-formed multi-species biofilm trials showed no antimicrobial effect of L-PRF exudate against P. gingivalis or other species. Our findings showed that L-PRF exudate may release peroxide and peptides, which may be responsible for its antimicrobial effect against P. gingivalis. In addition, L-PRF exudate had an antimicrobial effect against P. gingivalis in an in vitro developing multi-species biofilm.

Published
2021

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