Search

Your search keyword '"Krishna G. Prajapati"' showing total 2 results
Start Over Author "Krishna G. Prajapati" Journal frontiers in cellular and infection microbiology
2 results on '"Krishna G. Prajapati"'

1. Assessment of an Antibody-in-Lymphocyte Supernatant Assay for the Etiological Diagnosis of Pneumococcal Pneumonia in Children

Author

Michael J. Carter, Pallavi Gurung, Claire Jones, Shristy Rajkarnikar, Rama Kandasamy, Meeru Gurung, Stephen Thorson, Madhav C. Gautam, Krishna G. Prajapati, Bibek Khadka, Anju Maharjan, Julian C. Knight, David R. Murdoch, Thomas C. Darton, Merryn Voysey, Brian Wahl, Katherine L. O'Brien, Sarah Kelly, Imran Ansari, Ganesh Shah, Nina Ekström, Merit Melin, Andrew J. Pollard, Dominic F. Kelly, and Shrijana Shrestha

Subjects
pneumococcus (Streptococcus pneumoniae), pneumonia, antibodies, diagnostic test (MeSH), lymphocytes, Microbiology, QR1-502
Abstract

New diagnostic tests for the etiology of childhood pneumonia are needed. We evaluated the antibody-in-lymphocyte supernatant (ALS) assay to detect immunoglobulin (Ig) G secretion from ex vivo peripheral blood mononuclear cell (PBMC) culture, as a potential diagnostic test for pneumococcal pneumonia. We enrolled 348 children with pneumonia admitted to Patan Hospital, Kathmandu, Nepal between December 2015 and September 2016. PBMCs sampled from participants were incubated for 48 h before harvesting of cell culture supernatant (ALS). We used a fluorescence-based multiplexed immunoassay to measure the concentration of IgG in ALS against five conserved pneumococcal protein antigens. Of children with pneumonia, 68 had a confirmed etiological diagnosis: 12 children had pneumococcal pneumonia (defined as blood or pleural fluid culture-confirmed; or plasma CRP concentration ≥60 mg/l and nasopharyngeal carriage of serotype 1 pneumococci), and 56 children had non-pneumococcal pneumonia. Children with non-pneumococcal pneumonia had either a bacterial pathogen isolated from blood (six children); or C-reactive protein

Published
2020
Full Text
View/download PDF

2. Assessment of an Antibody-in-Lymphocyte Supernatant Assay for the Etiological Diagnosis of Pneumococcal Pneumonia in Children

Author

Michael J. Carter, Pallavi Gurung, Claire Jones, Shristy Rajkarnikar, Rama Kandasamy, Meeru Gurung, Stephen Thorson, Madhav C. Gautam, Krishna G. Prajapati, Bibek Khadka, Anju Maharjan, Julian C. Knight, David R. Murdoch, Thomas C. Darton, Merryn Voysey, Brian Wahl, Katherine L. O'Brien, Sarah Kelly, Imran Ansari, Ganesh Shah, Nina Ekström, Merit Melin, Andrew J. Pollard, Dominic F. Kelly, and Shrijana Shrestha

Subjects
0301 basic medicine, Serotype, Male, lcsh:QR1-502, lcsh:Microbiology, pneumococcus (Streptococcus pneumoniae), Cellular and Infection Microbiology, diagnostic test (MeSH), antibodies, Lymphocytes, Prospective Studies, Child, Pathogen, Original Research, biology, Antibodies, Bacterial, 3. Good health, Infectious Diseases, C-Reactive Protein, Streptococcus pneumoniae, Child, Preschool, Pneumococcal pneumonia, Female, Antibody, Microbiology (medical), Adolescent, 030106 microbiology, Immunology, Immunologic Tests, Microbiology, Peripheral blood mononuclear cell, Sensitivity and Specificity, Virus, 03 medical and health sciences, Antigen, Bacterial Proteins, Nepal, medicine, pneumonia, Humans, Antigens, Bacterial, business.industry, Diagnostic Tests, Routine, Infant, Pneumonia, Pneumococcal, medicine.disease, respiratory tract diseases, Pneumonia, 030104 developmental biology, Immunoglobulin G, biology.protein, Leukocytes, Mononuclear, business
Abstract

New diagnostic tests for the etiology of childhood pneumonia are needed. We evaluated the antibody-in-lymphocyte supernatant (ALS) assay to detect immunoglobulin (Ig) G secretion from ex vivo peripheral blood mononuclear cell (PBMC) culture, as a potential diagnostic test for pneumococcal pneumonia. We enrolled 348 children with pneumonia admitted to Patan Hospital, Kathmandu, Nepal between December 2015 and September 2016. PBMCs sampled from participants were incubated for 48 h before harvesting of cell culture supernatant (ALS). We used a fluorescence-based multiplexed immunoassay to measure the concentration of IgG in ALS against five conserved pneumococcal protein antigens. Of children with pneumonia, 68 had a confirmed etiological diagnosis: 12 children had pneumococcal pneumonia (defined as blood or pleural fluid culture-confirmed; or plasma CRP concentration ≥60 mg/l and nasopharyngeal carriage of serotype 1 pneumococci), and 56 children had non-pneumococcal pneumonia. Children with non-pneumococcal pneumonia had either a bacterial pathogen isolated from blood (six children); or C-reactive protein

Published
2019

Catalog

Books, media, physical & digital resources
See catalog results