1. Infiltration by CXCL10 Secreting Macrophages Is Associated With Antitumor Immunity and Response to Therapy in Ovarian Cancer Subtypes.
- Author
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Ardighieri L, Missale F, Bugatti M, Gatta LB, Pezzali I, Monti M, Gottardi S, Zanotti L, Bignotti E, Ravaggi A, Tognon G, Odicino F, Calza S, Missolo-Koussou Y, Ries CH, Helft J, and Vermi W
- Subjects
- Aged, Antigens, CD metabolism, Antigens, Differentiation, Myelomonocytic metabolism, CD3 Complex metabolism, Carcinoma drug therapy, Carcinoma genetics, Carcinoma immunology, Cells, Cultured, Chemokine CXCL10 genetics, Drug Resistance, Neoplasm, Female, Humans, Interferon Regulatory Factor-1 genetics, Interferon Regulatory Factor-1 metabolism, Lymphocytes, Tumor-Infiltrating immunology, Membrane Glycoproteins genetics, Membrane Glycoproteins metabolism, Middle Aged, Neoplasms, Cystic, Mucinous, and Serous drug therapy, Neoplasms, Cystic, Mucinous, and Serous genetics, Neoplasms, Cystic, Mucinous, and Serous immunology, Ovarian Neoplasms drug therapy, Ovarian Neoplasms genetics, Ovarian Neoplasms immunology, Phenotype, Prognosis, Receptors, Cell Surface metabolism, Receptors, Immunologic genetics, Receptors, Immunologic metabolism, STAT1 Transcription Factor genetics, STAT1 Transcription Factor metabolism, Tumor-Associated Macrophages immunology, Carcinoma metabolism, Chemokine CXCL10 metabolism, Lymphocytes, Tumor-Infiltrating metabolism, Neoplasms, Cystic, Mucinous, and Serous metabolism, Ovarian Neoplasms metabolism, Tumor Microenvironment, Tumor-Associated Macrophages metabolism
- Abstract
Ovarian carcinomas (OCs) are poorly immunogenic and immune checkpoint inhibitors (ICIs) have offered a modest benefit. In this study, high CD3
+ T-cells and CD163+ tumor-associated macrophages (TAMs) densities identify a subgroup of immune infiltrated high-grade serous carcinomas (HGSCs) with better outcomes and superior response to platinum-based therapies. On the contrary, in most clear cell carcinomas (CCCs) showing poor prognosis and refractory to platinum, a high TAM density is associated with low T cell frequency. Immune infiltrated HGSC are characterized by the 30-genes signature (OC-IS30 ) covering immune activation and IFNγ polarization and predicting good prognosis (n = 312, TCGA). Immune infiltrated HGSC contain CXCL10 producing M1-type TAM (IRF1+ pSTAT1Y701+ ) in close proximity to T-cells. A fraction of these M1-type TAM also co-expresses TREM2. M1-polarized TAM were barely detectable in T-cell poor CCC, but identifiable across various immunogenic human cancers. Single cell RNA sequencing data confirm the existence of a tumor-infiltrating CXCL10+ IRF1+ STAT1+ M1-type TAM overexpressing antigen processing and presentation gene programs. Overall, this study highlights the clinical relevance of the CXCL10+ IRF1+ STAT1+ macrophage subset as biomarker for intratumoral T-cell activation and therefore offers a new tool to select patients more likely to respond to T-cell or macrophage-targeted immunotherapies., Competing Interests: SG was employed by Diatech Pharmacogenetics srl and CR was employed by Dr Carola Ries Consulting. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest., (Copyright © 2021 Ardighieri, Missale, Bugatti, Gatta, Pezzali, Monti, Gottardi, Zanotti, Bignotti, Ravaggi, Tognon, Odicino, Calza, Missolo-Koussou, Ries, Helft and Vermi.)- Published
- 2021
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