1. A Cross-Protective Vaccine Against 4b and 1/2b Listeria monocytogenes
- Author
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Fanzeng Meng, Tengfei Zhu, Hao Yao, Zhiting Ling, Youwei Feng, Guo Li, Jing Li, Xinyu Sun, Jiaqi Chen, Chuang Meng, Xin’an Jiao, and Yuelan Yin
- Subjects
Listeria monocytogenes ,attenuated vaccine ,cellular immune response ,cross protection ,humoral immune response ,Microbiology ,QR1-502 - Abstract
Listeria monocytogenes (Lm) is a foodborne zoonotic pathogen that causes listeriosis with a mortality rate of 20–30%. Serovar 4b and 1/2b isolates account for most of listeriosis outbreaks, however, no listeriosis vaccine is available for either prophylactic or therapeutic use. Here, we developed a triple-virulence-genes deletion vaccine strain, and evaluated its safety, immunogenicity, and cross-protective efficiency. The virulence of NTSNΔactA/plcB/orfX was reduced 794-folds compared with the parental strain. Additionally, it was completely eliminated in mice at day 7 post infection and no obvious pathological changes were observed in the organs of mice after prime-boost immunization for 23 days. These results proved that the safety of the Lm vaccine strain remarkably increased. More importantly, the NTSNΔactA/plcB/orfX strain stimulated higher anti-Listeriolysin O (LLO) antibodies, induced significantly higher expression of IFN-γ, TNF-α, IL-17, and IL-6 than the control group, and afforded 100% protection against serovar 4b and 1/2b challenges. Taken together, our research demonstrates that the triple-genes-deletion vaccine has high safety, can elicit strong Th1 type immune response, and affords efficient cross-protection against two serovar Lm strains. It is a promising vaccine for prevention of listeriosis.
- Published
- 2020
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