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Start Over Descriptor "food pathogen" Journal frontiers in microbiology
4 results on '"food pathogen"'

1. The combination of allicin with domiphen is effective against microbial biofilm formation.

Author

Shang Li, Yutong Wang, Geweirong Xu, Yuqing Xu, Cuiyan Fu, Quanlin Zhao, Linjie Xu, Xinzhou Jia, Yumeng Zhang, Yi Liu, and Jiaju Qiao

Subjects
CANDIDA albicans, ESCHERICHIA coli, BIOFILMS, MICROBIAL growth, GENTIAN violet, SURFACES (Technology)
Abstract

Background: Microorganisms in biofilms are particularly difficult to control because of their increased survival and antibiotic resistance. Allicin and domiphen were employed to inhibit the microbial growth and biofilm formation of Staphylococcus aureus, Escherichia coli, and Candida albicans strains. Methods: Broth microdilution method and checkerboard assay were conducted to determine the efficacy of allicin combined with domiphen against S. aureus, E. coli, and C. albicans. Microbial biofilm formation was measured using the crystal violet staining method and fluorescence microscopy. And the total viable count of the biofilm cells on material surface after the treatment with antimicrobial reagents was calculated with the plate count technique. Results: The two drugs showed synergistic effects against the pathogens with a fractional bactericidal concentration of less than 0.38. The combination of 64 µg/mL allicin with 1 µg/mL domiphen dispersed over 50% of the biofilm mass of S. aureus, E. coli, and C. albicans. In addition, the drug combination reduced the total viable counts of E. coli and C. albicans biofilm cells on stainless steel and polyethylene surfaces by more than 102 CFU/mL. Conclusion: The combination of allicin and domiphen is an effective strategy for efficiently decreasing biofilms formation on various industrial materials surfaces. [ABSTRACT FROM AUTHOR]

Published
2024
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2. Water-Soluble Ruthenium (II) Complex Derived From Optically Pure Limonene and Its Microencapsulation Are Efficient Tools Against Bacterial Food Pathogen Biofilms: Escherichia coli , Staphylococcus aureus , Enteroccocus faecalis , and Listeria monocytogenes

Author

Khelissa, Simon, El Fannassi, Yousra, Mechmechani, Samah, Alhuthali, Sakhr, El Amrani, Mohamed Amin, Gharsallaoui, Adem, Barras, Alexandre, and Chihib, Nour-Eddine

Subjects
LISTERIA monocytogenes, FOOD pathogens, RUTHENIUM, ESCHERICHIA coli, STAPHYLOCOCCUS aureus, BACTERIAL cell membranes, LIMONENE
Abstract

Bioactive aminooxime ligands based on optically pure (R)-limonene have been synthesized in two steps. Their ruthenium (II) cationic water-soluble complex was prepared by a reaction between dichloro (para-cymene) ruthenium (II) dimers and aminooxime ligands in a 1:2 molar ratio. Antibacterial and antibiofilm activities of the synthetized complex were assessed against Escherichia coli , Staphylococcus aureus , Listeria monocytogenes , and Enterococcus faecalis. The results revealed that the ruthenium (II) complex has higher antibacterial and antibiofilm activities in comparison with free ligands or the enantiopure (R)-limonene. Moreover, microencapsulation of this complex reduced its cytotoxicity and improved their minimum inhibitory concentration and antibiofilm activity toward the considered bacteria. The ruthenium (II) complex targets the bacterial cell membrane, which leads to rapid leakage of intracellular potassium. Our study suggests that the developed ruthenium (II) complexes could be useful as an alternative to conventional disinfectants. [ABSTRACT FROM AUTHOR]

Published
2021
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3. Water-Soluble Ruthenium (II) Complex Derived From Optically Pure Limonene and Its Microencapsulation Are Efficient Tools Against Bacterial Food Pathogen Biofilms: Escherichia coli, Staphylococcus aureus, Enteroccocus faecalis, and Listeria monocytogenes

Author

Nour-Eddine Chihib, Sakhr Alhuthali, Adem Gharsallaoui, Samah Mechmechani, Simon Khelissa, Alexandre Barras, Yousra El Fannassi, Mohamed Amin El Amrani, Unité Matériaux et Transformations - UMR 8207 (UMET), Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Centrale Lille Institut (CLIL), Université Abdelmalek Essaâdi (UAE), Laboratoire d'automatique, de génie des procédés et de génie pharmaceutique (LAGEPP), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-École Supérieure Chimie Physique Électronique de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut d’Électronique, de Microélectronique et de Nanotechnologie - UMR 8520 (IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), NanoBioInterfaces - IEMN (NBI - IEMN), Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA), This work was carried out within the framework of TOUBKAL PROJECT No. 43716SB. The authors thank Campus France and the Partenariats Hubert Curien program and Imperial Open Access Fund for their financial support. This program was carried out between Abdelmalek Essaadi University, Faculty of Sciences, Tetouan, Morocco and Lille University, Lille, France., Centrale Lille-Institut de Chimie du CNRS (INC)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Centre National de la Recherche Scientifique (CNRS), Université catholique de Lille (UCL)-Université catholique de Lille (UCL), and Université catholique de Lille (UCL)-Université catholique de Lille (UCL)-Centrale Lille-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Université Polytechnique Hauts-de-France (UPHF)-JUNIA (JUNIA)

Subjects
Microbiology (medical), antibacterial agent, complexation, chemistry.chemical_element, biofilm removal, medicine.disease_cause, Microbiology, Bacterial cell structure, Enterococcus faecalis, biofilm, [SPI]Engineering Sciences [physics], 03 medical and health sciences, Minimum inhibitory concentration, Listeria monocytogenes, [SDV.IDA]Life Sciences [q-bio]/Food engineering, medicine, [CHIM.COOR]Chemical Sciences/Coordination chemistry, 0502 Environmental Science and Management, 0503 Soil Sciences, Escherichia coli, 030304 developmental biology, Antibacterial agent, 0303 health sciences, ruthenium (II), biology, 030306 microbiology, Chemistry, biology.organism_classification, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, Combinatorial chemistry, QR1-502, Ruthenium, limonene, microencapsulation, food pathogen, Bacteria, 0605 Microbiology
Abstract

Bioactive aminooxime ligands based on optically pure (R)-limonene have been synthesized in two steps. Their ruthenium (II) cationic water-soluble complex was prepared by a reaction between dichloro (para-cymene) ruthenium (II) dimers and aminooxime ligands in a 1:2 molar ratio. Antibacterial and antibiofilm activities of the synthetized complex were assessed against Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis. The results revealed that the ruthenium (II) complex has higher antibacterial and antibiofilm activities in comparison with free ligands or the enantiopure (R)-limonene. Moreover, microencapsulation of this complex reduced its cytotoxicity and improved their minimum inhibitory concentration and antibiofilm activity toward the considered bacteria. The ruthenium (II) complex targets the bacterial cell membrane, which leads to rapid leakage of intracellular potassium. Our study suggests that the developed ruthenium (II) complexes could be useful as an alternative to conventional disinfectants.

Published
2021
Full Text
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4. Water-Soluble Ruthenium (II) Complex Derived From Optically Pure Limonene and Its Microencapsulation Are Efficient Tools Against Bacterial Food Pathogen Biofilms

Author

Simon, Khelissa, Yousra, El Fannassi, Samah, Mechmechani, Sakhr, Alhuthali, Mohamed Amin, El Amrani, Adem, Gharsallaoui, Alexandre, Barras, and Nour-Eddine, Chihib

Subjects
ruthenium (II), antibacterial agent, complexation, limonene, microencapsulation, biofilm removal, Microbiology, biofilm, food pathogen, Original Research
Abstract

Bioactive aminooxime ligands based on optically pure (R)-limonene have been synthesized in two steps. Their ruthenium (II) cationic water-soluble complex was prepared by a reaction between dichloro (para-cymene) ruthenium (II) dimers and aminooxime ligands in a 1:2 molar ratio. Antibacterial and antibiofilm activities of the synthetized complex were assessed against Escherichia coli, Staphylococcus aureus, Listeria monocytogenes, and Enterococcus faecalis. The results revealed that the ruthenium (II) complex has higher antibacterial and antibiofilm activities in comparison with free ligands or the enantiopure (R)-limonene. Moreover, microencapsulation of this complex reduced its cytotoxicity and improved their minimum inhibitory concentration and antibiofilm activity toward the considered bacteria. The ruthenium (II) complex targets the bacterial cell membrane, which leads to rapid leakage of intracellular potassium. Our study suggests that the developed ruthenium (II) complexes could be useful as an alternative to conventional disinfectants.

Published
2021

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