Search

Your search keyword '"Archana Sachdev"' showing total 2 results
Start Over Author "Archana Sachdev" Journal frontiers in plant science
2 results on '"Archana Sachdev"'

1. Development and Evaluation of Low Phytic Acid Soybean by siRNA Triggered Seed Specific Silencing of Inositol Polyphosphate 6-/3-/5-Kinase Gene

Author

Mansi Punjabi, Navneeta Bharadvaja, Monica Jolly, Anil Dahuja, and Archana Sachdev

Subjects
soybean, low phytic acid, inositol polyphosphate 6-/3-/5-kinase, RNAi silencing, seed-specific, Agrobacterium-mediated transformation, Plant culture, SB1-1110
Abstract

Soybean is one of the leading oilseed crop in the world and is showing a remarkable surge in its utilization in formulating animal feeds and supplements. Its dietary consumption, however, is incongruent with its existing industrial demand due to the presence of anti-nutritional factors in sufficiently large amounts. Phytic acid in particular raises concern as it causes a concomitant loss of indigestible complexed minerals and charged proteins in the waste and results in reduced mineral bioavailability in both livestock and humans. Reducing the seed phytate level thus seems indispensable to overcome the nutritional menace associated with soy grain consumption. In order to conceive our objective we designed and expressed a inositol polyphosphate 6-/3-/5-kinase gene-specific RNAi construct in the seeds of Pusa-16 soybean cultivar. We subsequently conducted a genotypic, phenotypic and biochemical analysis of the developed putative transgenic populations and found very low phytic acid levels, moderate accumulation of inorganic phosphate and elevated mineral content in some lines. These low phytic acid lines did not show any reduction in seedling emergence and displayed an overall good agronomic performance.

Published
2018
Full Text
View/download PDF

2. Regulation of Isoflavone Biosynthesis by miRNAs in Two Contrasting Soybean Genotypes at Different Seed Developmental Stages

Author

Sanjeev Kumar, Anil Dahuja, Om Prakash Gupta, Archana Sachdev, T. Vinutha, Shelly Praveen, and Deepti Nigam

Subjects
0301 basic medicine, Riboregulator, Genetics, 4-Coumarate-CoA ligase, RA-PCR, Genistein, Glycitein, Plant Science, Biology, Isoflavones, genistein, 03 medical and health sciences, chemistry.chemical_compound, 030104 developmental biology, chemistry, isoflavone 7-O-glucosyltransferase, Gene expression, microRNA, KEGG, daidzein, Gene, glycitein, Original Research, miRNA
Abstract

Owing to the presence of nutritionally important, health-promoting bioactive compounds, especially isoflavones, soybean has acquired the status of a functional food. miRNAs are tiny riboregulator of gene expression by either decreasing and/or increasing the expression of their corresponding target genes. Despite several works on identification and functional characterization of plant miRNAs, the role of miRNAs in the regulation of isoflavones metabolism is still a virgin field. In the present study, we identified a total of 31 new miRNAs along with their 245 putative target genes from soybean seed-specific ESTs using computational approach. The Kyoto Encyclopedia of Genes and Genomes pathway analyses indicated that miRNA putatively regulates metabolism and genetic information processing. Out of that, a total of 5 miRNAs (Gma-miRNA12, Gma-miRNA24, Gma-miRNA26, Gma-miRNA28, and Gma-miRNA29) were predicted and validated for their probable role during isoflavone biosynthesis. We also validated their five target genes using RA-PCR, which is as good as 5'RLM-RACE. Temporal regulation [35 days after flowering, 45, 55, and 65 DAF] of miRNAs and their targets showed differential expression schema. Differential expression of Gma-miR26 and Gma-miRNA28 along with their corresponding target genes (Glyma.10G197900 and Glyma.09G127200) showed a direct relationship with the total isoflavone content. Therefore, understanding the miRNA-based genetic regulation of isoflavone pathway would assist in selection and manipulation to get high-performing soybean genotypes with better isoflavone yield.

Published
2017

Catalog

Books, media, physical & digital resources
See catalog results