7 results on '"Lu-Ning Liu"'
Search Results
2. Editorial: Structure and Function of Chloroplasts - Volume II
- Author
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Yan Lu, Lu-Ning Liu, Rebecca L. Roston, Jürgen Soll, and Hongbo Gao
- Subjects
chloroplast ,envelope ,thylakoid ,protein import ,photosynthesis ,Plant culture ,SB1-1110 - Published
- 2020
- Full Text
- View/download PDF
Catalog
3. Responses of Membranes and the Photosynthetic Apparatus to Salt Stress in Cyanobacteria
- Author
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Wenjing Yang, Fang Wang, Lu-Ning Liu, and Na Sui
- Subjects
cyanobacteria ,membranes lipids ,photosynthetic apparatus ,photosynthesis ,salt stress ,Plant culture ,SB1-1110 - Abstract
Cyanobacteria are autotrophs whose photosynthetic process is similar to that of higher plants, although the photosynthetic apparatus is slightly different. They have been widely used for decades as model systems for studying the principles of photosynthesis, especially the effects of environmental stress on photosynthetic activities. Salt stress, which is the most common abiotic stress in nature, combines ionic and osmotic stresses. High cellular ion concentrations and osmotic stress can alter normal metabolic processes and photosynthesis. Additionally, salt stress increases the intracellular reactive oxygen species (ROS) contents. Excessive amounts of ROS will damage the photosynthetic apparatus, inhibit the synthesis of photosystem-related proteins, including the D1 protein, and destroy the thylakoid membrane structure, leading to inhibited photosynthesis. In this review, we mainly introduce the effects of salt stress on the cyanobacterial membranes and photosynthetic apparatus. We also describe specific salt tolerance mechanisms. A thorough characterization of the responses of membranes and photosynthetic apparatus to salt stress may be relevant for increasing agricultural productivity. more...
- Published
- 2020
- Full Text
- View/download PDF
4. Photosynthetic Regulation Under Salt Stress and Salt-Tolerance Mechanism of Sweet Sorghum
- Author
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Zhen Yang, Jin-Lu Li, Lu-Ning Liu, Qi Xie, and Na Sui
- Subjects
sweet sorghum ,salt-tolerance mechanism ,Na+ exclusion ,photosynthesis ,sugar content ,Plant culture ,SB1-1110 - Abstract
Sweet sorghum is a C4 crop with the characteristic of fast-growth and high-yields. It is a good source for food, feed, fiber, and fuel. On saline land, sweet sorghum can not only survive, but increase its sugar content. Therefore, it is regarded as a potential source for identifying salt-related genes. Here, we review the physiological and biochemical responses of sweet sorghum to salt stress, such as photosynthesis, sucrose synthesis, hormonal regulation, and ion homeostasis, as well as their potential salt-resistance mechanisms. The major advantages of salt-tolerant sweet sorghum include: 1) improving the Na+ exclusion ability to maintain ion homeostasis in roots under salt-stress conditions, which ensures a relatively low Na+ concentration in shoots; 2) maintaining a high sugar content in shoots under salt-stress conditions, by protecting the structures of photosystems, enhancing photosynthetic performance and sucrose synthetase activity, as well as inhibiting sucrose degradation. To study the regulatory mechanism of such genes will provide opportunities for increasing the salt tolerance of sweet sorghum by breeding and genetic engineering. more...
- Published
- 2020
- Full Text
- View/download PDF
5. Engineering and Modulating Functional Cyanobacterial CO2-Fixing Organelles
- Author
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Yi Fang, Fang Huang, Matthew Faulkner, Qiuyao Jiang, Gregory F. Dykes, Mengru Yang, and Lu-Ning Liu
- Subjects
bacterial microcompartment ,carboxysome ,carbon fixation ,cyanobacteria ,encapsulation ,Rubisco ,Plant culture ,SB1-1110 - Abstract
Bacterial microcompartments (BMCs) are proteinaceous organelles widespread among bacterial phyla and provide a means for compartmentalizing specific metabolic pathways. They sequester catalytic enzymes from the cytoplasm, using an icosahedral proteinaceous shell with selective permeability to metabolic molecules and substrates, to enhance metabolic efficiency. Carboxysomes were the first BMCs discovered and their unprecedented capacity of CO2 fixation allows cyanobacteria to make a significant contribution to global carbon fixation. There is an increasing interest in utilizing synthetic biology to construct synthetic carboxysomes in new hosts, i.e., higher plants, to enhance carbon fixation and productivity. Here, we report the construction of a synthetic operon of the β-carboxysome from the cyanobacterium Synechococcus elongatus PCC7942 to generate functional β-carboxysome-like structures in Escherichia coli. The protein expression, structure, assembly, and activity of synthetic β-carboxysomes were characterized in depth using confocal, electron and atomic force microscopy, proteomics, immunoblot analysis, and enzymatic assays. Furthermore, we examined the in vivo interchangeability of β-carboxysome building blocks with other BMC components. To our knowledge, this is the first production of functional β-carboxysome-like structures in heterologous organisms. It provides important information for the engineering of fully functional carboxysomes and CO2-fixing modules in higher plants. The study strengthens our synthetic biology toolbox for generating BMC-based organelles with tunable activities and new scaffolding biomaterials for metabolic improvement and molecule delivery. more...
- Published
- 2018
- Full Text
- View/download PDF
6. Responses of Membranes and the Photosynthetic Apparatus to Salt Stress in Cyanobacteria
- Author
-
Fang Wang, Na Sui, Lu-Ning Liu, and Wenjing Yang
- Subjects
0106 biological sciences ,0301 basic medicine ,Cyanobacteria ,Osmotic shock ,Mini Review ,Salt (chemistry) ,Plant Science ,lcsh:Plant culture ,Photosynthesis ,cyanobacteria ,01 natural sciences ,membranes lipids ,03 medical and health sciences ,photosynthetic apparatus ,lcsh:SB1-1110 ,Autotroph ,salt stress ,chemistry.chemical_classification ,photosynthesis ,biology ,Chemistry ,Abiotic stress ,biology.organism_classification ,030104 developmental biology ,Membrane ,Thylakoid ,Biophysics ,010606 plant biology & botany - Abstract
Cyanobacteria are autotrophs whose photosynthetic process is similar to that of higher plants, although the photosynthetic apparatus is slightly different. They have been widely used for decades as model systems for studying the principles of photosynthesis, especially the effects of environmental stress on photosynthetic activities. Salt stress, which is the most common abiotic stress in nature, combines ionic and osmotic stresses. High cellular ion concentrations and osmotic stress can alter normal metabolic processes and photosynthesis. Additionally, salt stress increases the intracellular reactive oxygen species (ROS) contents. Excessive amounts of ROS will damage the photosynthetic apparatus, inhibit the synthesis of photosystem-related proteins, including the D1 protein, and destroy the thylakoid membrane structure, leading to inhibited photosynthesis. In this review, we mainly introduce the effects of salt stress on the cyanobacterial membranes and photosynthetic apparatus. We also describe specific salt tolerance mechanisms. A thorough characterization of the responses of membranes and photosynthetic apparatus to salt stress may be relevant for increasing agricultural productivity. more...
- Published
- 2020
- Full Text
- View/download PDF
7. Engineering and Modulating Functional Cyanobacterial CO2-Fixing Organelles.
- Author
-
Yi Fang, Fang Huang, Faulkner, Matthew, Qiuyao Jiang, Dykes, Gregory F., Mengru Yang, and Lu-Ning Liu
- Subjects
PLANT organelles ,CYANOBACTERIA ,CARBON fixation - Abstract
Bacterial microcompartments (BMCs) are proteinaceous organelles widespread among bacterial phyla and provide a means for compartmentalizing specific metabolic pathways. They sequester catalytic enzymes from the cytoplasm, using an icosahedral proteinaceous shell with selective permeability to metabolic molecules and substrates, to enhance metabolic efficiency. Carboxysomes were the first BMCs discovered and their unprecedented capacity of CO
2 fixation allows cyanobacteria to make a significant contribution to global carbon fixation. There is an increasing interest in utilizing synthetic biology to construct synthetic carboxysomes in new hosts, i.e., higher plants, to enhance carbon fixation and productivity. Here, we report the construction of a synthetic operon of the b-carboxysome from the cyanobacterium Synechococcus elongatus PCC7942 to generate functional β-carboxysome-like structures in Escherichia coli. The protein expression, structure, assembly, and activity of synthetic b-carboxysomes were characterized in depth using confocal, electron and atomic force microscopy, proteomics, immunoblot analysis, and enzymatic assays. Furthermore, we examined the in vivo interchangeability of β-carboxysome building blocks with other BMC components. To our knowledge, this is the first production of functional b-carboxysomelike structures in heterologous organisms. It provides important information for the engineering of fully functional carboxysomes and CO2 -fixing modules in higher plants. The study strengthens our synthetic biology toolbox for generating BMC-based organelles with tunable activities and new scaffolding biomaterials for metabolic improvement and molecule delivery. [ABSTRACT FROM AUTHOR] more...- Published
- 2018
- Full Text
- View/download PDF
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