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• A biocrude was collected from the hydrothermal liquefaction of algae slurry. • LBV of microalgae oil/RP-3/air mixtures were obtained in a constant volume chamber. • The effects of temperature, pressure, microalgae oil addition on LBV were analyzed. • By fitting experimental data, an empirical formula of LBV with factors was obtained. In the present work, algae-derived biocrude was collected from the hydrothermal liquefaction of algae slurry, and the mixtures of algae-derived biocrude and RP-3 aviation kerosene was used as aviation substitute fuel named as microalgae oil/RP-3 blend. In order to understand the laminar burning velocity (LBV) of microalgae oil/RP-3, experiments have been carried out in a constant volume chamber (CVC) to investigate the effects of initial temperature, initial pressure and microalgae oil addition on the LBV of microalgae oil/RP-3 blend over a wide equivalence ratio range from 0.8 to 1.4. Experimental result shows that with the increase of initial temperature and microalgae oil addition, the LBV of microalgae oil/RP-3 mixture increases, while with the increase of initial pressure the LBV of microalgae oil/RP-3 mixture decreases. According to the method of Metghachi, the relationship between the LBV and its influence factors was obtained, the exponents of α T (ϕ) , β P (ϕ) and γ R (ϕ) indicate that the change of initial temperature and pressure have less effect on LBV around equivalence ratio of 1.1 compared with other equivalence ratio conditions, while the change of microalgae oil addition has more significant effect to improve LBV at equivalence ratio of 1.1. [ABSTRACT FROM AUTHOR]