Search

In this study, lignite was degraded using a purified esterase from Penicillium decumbens P6 for the first time. The esterase was purified using ammonium sulfate precipitation, anion exchange and gel filtration chromatography. The recovery and purification yield of the enzyme were 15% and 83 folds, respectively. The molecular weight of the purified enzyme was about 45 kDa. Both crude and purified esterases were studied for lignite depolymerization. The tendency of depolymerization by crude enzyme was consistent with the enzyme secreted in the medium. Along with the increased purified esterase concentration from 8 to 50 mg/ml, A 450 value increased from 0.38 to 2.08. The contribution of esterase to the depolymerization was about 40% in the crude supernatant. Compared with aHA (crude lignite humic acid), bHA (esterase degraded lignite humic acid) has a lower percentage of aromatic carbon and ester groups, but a higher percentage of aliphatic carbon. bHA can promote the growth of asparagus lettuce. The results demonstrated that lignite was depolymerized by the purified esterase and evidenced the potential of esterase application in conversion of lignite into compounds with high bioactivities. [ABSTRACT FROM AUTHOR]

Abstract: The process involved in lignite degradation/solubilization by Penicillium decumbens P6 was studied. P6 secreted some unknown alkaline substance and increased the pH of the medium as it grew. Meanwhile, it excreted some surfactants that might be involved in lignite degradation. The most important aspect in this study was that P6 could excrete some extracellular enzymes that might participate in the lignite degradation. With the induction of lignite, the activities of peroxidases and esterases, two classes of enzymes capable of attacking lignite, were increased in the cell-free filtrate of the culture. [Copyright &y& Elsevier]