1. Expression of a Nicotiana tabacum pathogen-induced gene is involved in the susceptibility to black shank.
- Author
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Portieles, Roxana, Canales, Eduardo, Chacon, Osmani, Silva, Yussuan, Hernández, Ingrid, López, Yunior, Rodríguez, Mayra, Terauchi, Ryohei, Hideo Matsumura, Borroto, Carlos, Walton, Jonathan D., Santos, Ramon, and Borrás-Hidalgo, Orlando
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GENETIC engineering of tobacco , *PLANT genetic engineering , *DISEASE susceptibility , *PATHOGENIC viruses , *NICOTIANA - Abstract
Many host genes induced during compatible plant-pathogen interactions constitute targets of pathogen virulence factors that act to suppress host defenses. In order to identify Nicotiana tabacum L. genes for pathogen-induced proteins involved in susceptibility to the oomycete Phytophthora parasitica var. nicotianae, we used SuperSAGE technology combined with next-generation sequencing to identify transcripts that were differentially upregulated during a compatible interaction. We identified a pathogen-induced gene (NtPIP) that was rapidly induced only during the compatible interaction. Virus-induced gene silencing of NtPIP reduced the susceptibility of N. tabacum to P. parasitica var. nicotianae. Additionally, transient expression of NtPIP in the resistant species Nicotiana megalosiphon Van Heurck & Mull. Arg. compromised the resistance to P. parasitica var. nicotianae. This pathogen-induced protein is therefore a positive regulator of the susceptibility response against an oomycete pathogen in tobacco. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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