Search

Your search keyword '"Brown, Daren W."' showing total 17 results
Start Over Author "Brown, Daren W." Journal fungal genetics and biology
17 results on '"Brown, Daren W."'

17. A Genetic and Biochemical Approach to Study Trichothecene Diversity in Fusarium sporotrichioidesand Fusarium graminearum

Author

Brown, Daren W., McCormick, Susan P., Alexander, Nancy J., Proctor, Robert H., and Desjardins, Anne E.

Abstract

The trichothecenes T-2 toxin and deoxynivalenol (DON) are natural fungal products that are toxic to both animals and plants. Their importance in the pathogenicity of Fusariumspp. on crop plants has inspired efforts to understand the genetic and biochemical mechanisms leading to trichothecene synthesis. In order to better understand T-2 toxin biosynthesis by Fusarium sporotrichioidesand DON biosynthesis by F. graminearum,we compared the nucleotide sequence of the 23-kb core trichothecene gene cluster from each organism. This comparative genetic analysis allowed us to predict proteins encoded by two trichothecene genes, TRI9and TRI10,that had not previously been described from either Fusariumspecies. Differences in gene structure also were correlated with differences in the types of trichothecenes that the two species produce. Gene disruption experiments showed that F. sporotrichioides TRI7(FsTRI7) is required for acetylation of the oxygen on C-4 of T-2 toxin. Sequence analysis indicated that F. graminearum TRI7(FgTRI7) is nonfunctional. This is consistent with the fact that the FgTRI7product is not required for DON synthesis in F. graminearumbecause C-4 is not oxygenated.

Published
2001
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results