1. Hepatic cells derived from induced pluripotent stem cells of pigtail macaques support hepatitis C virus infection.
- Author
-
Sourisseau M, Goldman O, He W, Gori JL, Kiem HP, Gouon-Evans V, and Evans MJ
- Subjects
- Animals, Cell Line, Cells, Cultured, Hepatitis C pathology, Hepatitis C physiopathology, Hepatocytes pathology, Host-Pathogen Interactions genetics, Humans, Induced Pluripotent Stem Cells pathology, Occludin physiology, Tetraspanin 28 deficiency, Tetraspanin 28 physiology, Virus Internalization, Virus Replication physiology, Disease Models, Animal, Hepacivirus pathogenicity, Hepatitis C virology, Hepatocytes virology, Induced Pluripotent Stem Cells virology, Macaca nemestrina
- Abstract
The narrow species tropism of hepatitis C virus (HCV) limits animal studies. We found that pigtail macaque (Macaca nemestrina) hepatic cells derived from induced pluripotent stem cells support the entire HCV life cycle, although infection efficiency was limited by defects in the HCV cell entry process. This block was overcome by either increasing occludin expression, complementing the cells with human CD81, or infecting them with a strain of HCV with less restricted requirements for CD81. Using this system, we can modify viral and host cell genetics to make pigtail macaques a suitable, clinically relevant model for the study of HCV infection., (Copyright © 2013 AGA Institute. Published by Elsevier Inc. All rights reserved.)
- Published
- 2013
- Full Text
- View/download PDF