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G A A b st ra ct s histology. Methods: BE patients with and without early neoplasia underwent endoscopic resection (ER) of areas marked in-vivo with electrocoagulation markers (ECM). Subsequently ER specimens underwent additional ex-vivo marking with several different markers (ink, pin, ECM) followed by ex-vivo VLE scanning. Tissue blocks were carefully sectioned guided by the placed markers. After further histological processing a histopathology slide was sectioned from each block. When necessary, extensive sectioning of tissue blocks was performed in order to visualize all markers that were included in the tissue block on histology. All histopathology and VLE slides were evaluated by 2 researchers and considered a match if a) ≥ 2 markers were visible on both modalities and b) mucosal patterns aside from these markers matched on both histology and VLE. All slides were evaluated by an expert BE pathologist. Results: From 16 ER specimens (overall diagnosis: 7 non-dysplastic BE, 9 dysplastic BE (1 LGD, 4 HGD, 4 EAC)) 120 tissue blocks were sectioned of which 57 contained multiple markers and thus could potentially be matched with VLE. Based on several combinations of these markers in total 14 histology-VLE matches could ultimately be constructed. Markers that achieved the best yield of matches respectively were: invivo placed ECMs (8 matches with 12 markers), pins (7 with 11), and ink (4 with 5). Histopathological evaluation was not hindered by marker use. In this pilot study the last 6 ER specimens yielded 9/14 matches demonstrating a clear learning curve due to methodological improvements in marker placement and tissue block sectioning. Conclusion: One-to-one correlation of VLE and histology is complex but feasible. The groundwork laid in this study will provide high-quality histology-VLE correlations that will allow further research on VLE structures and VLE features of early neoplasia in BE.