1. Immunhistochemischer Nachweis des 'epidermal growth factor receptor (EGF-R)' an Paraffinschnitten von Mammakarzinomgeweben: Korrelationen und klinische Wertigkeit
- Author
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H.-G. Schnürch, Boris Tutschek, Engels K, F. Picard, U.J. Göhring, M. W. Beckmann, Anton Scharl, and Dieter Niederacher
- Subjects
Pathology ,medicine.medical_specialty ,biology ,Receptor expression ,Mammary gland ,Obstetrics and Gynecology ,medicine.disease ,medicine.anatomical_structure ,Breast cancer ,Epidermal growth factor ,Maternity and Midwifery ,medicine ,biology.protein ,Carcinoma ,Immunohistochemistry ,Epidermal growth factor receptor ,Lymph node ,hormones, hormone substitutes, and hormone antagonists - Abstract
Analyses of the level of expression of the epidermal growth factor receptor (EGF-R) of breast cancer tumours may add independent information about the prognosis for individual patients. Furthermore, the use of monoclonal antibodies directed against EGF-R as therapeutic tools (e.g., Mab 425) requires a reliable evaluation of the individual EGF-R content. Various analytical methods have been published, including (1) biochemical detectonn of EGF-R by a radiolabelled physiological ligand, (125I)EGF, (2) enzymatic analyses of EGF-R content (IEMA), (3) immunological analyses of EGF-R content with a monoclonal antibody (ELISA), and (4) immunohistochemical EGF-R detection. Studies with immunohistochemical analyses of EGF-R overexpression in formalin-fixed, paraffin-embedded tumour samples are rare. In a retrospective study, we examined the clinical data from 142 patients and the EGF-R expression in their formalin-fixed, paraffin-embedded tumour samples. The average follow-up was 69 months. EGF-R expression was compared to oestrogen (ER) and progesterone (PgR) receptor expression, histological grade, tumour size, lymph node metastases and menopause. 52 of 142 tumours were EGF-R positive. EGF-R overexpression correlated with high tumour grade, large tumours and elevated numbers of lymph node metastases. There was no significant correlation between ER or PgR and EGF-R expression. Determination of EGF-R overexpression revealed no significant difference in disease-free interval (DFI) or overall survival (OS). In this study, the determination of EGF-R in formalin-fixed, paraffin-embedded tumour samples proved feasible. Unfortunately, this did not add any additional information concerning DFI or OS.
- Published
- 1995
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