1. CIZ1 knockdown suppresses the proliferation of bladder cancer cells by inducing apoptosis.
- Author
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Chen X, Wang P, Wang S, Li J, Ou T, and Zeng X
- Subjects
- Aged, Cell Line, Cell Line, Tumor, Female, Gene Silencing, Genes, cdc, Humans, Male, Middle Aged, Signal Transduction, Urinary Bladder cytology, Urinary Bladder metabolism, Apoptosis genetics, Cell Proliferation genetics, Gene Knockdown Techniques, Nuclear Proteins genetics, Urinary Bladder Neoplasms pathology
- Abstract
Bladder cancer (BC) is the ninth most frequent malignancy and the thirteenth leading cause of cancer-related death worldwide. CDKN1A-interacting zinc finger protein 1 (CIZ1) is involved in the development of various cancers, while its role in BC remains unclear. In this study, we found that CIZ1 was up-regulated in BC tissues and cells. Knockdown of CIZ1 suppressed the proliferation and growth of T24 and 5637 cells. Apoptosis and caspase 3/7 activity were enhanced after CIZ1 silencing in both cells. At the molecular level, PCNA, Ki67, HIF-1α, survivin, CCND1 and CCNB1 were down-regulated, and caspase-3, p53, p21, caspase-9 were up-regulated by CIZ1 knockdown. This suggested that silencing of CIZ1 suppressed BC cell proliferation through inducing apoptosis and reducing cell cycle progression. Our study suggests that targeting CIZ1 maybe a potential therapeutic strategy for BC., (Copyright © 2019. Published by Elsevier B.V.)
- Published
- 2019
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