1. miR-181a regulate porcine preadipocyte differentiation by targeting TGFBR1.
- Author
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Zhang, Zhe, Gao, Yan, Xu, Ming-Qiang, Wang, Chang-Jiang, Fu, Xu-Huang, Liu, Jian-Bo, Han, Dong-Xu, Jiang, Hao, Yuan, Bao, and Zhang, Jia-Bao
- Subjects
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ADIPOGENESIS , *MICRORNA , *OLEIC acid , *PROTEIN expression , *WESTERN immunoblotting , *FLOW cytometry - Abstract
Abstract miRNAs have been shown to regulate a variety of biological process. It has been shown that miR-181a regulates porcine adipogenesis by targeting Tumor Necrosis Factor-α (TNF-α), but the overall functions of miR-181a in porcine preadipocyte differentiation remain unclear. This study aimed to explore the functions of miR-181a in porcine preadipocyte differentiation via the TGFβ/Smad pathway. The TargetScan program was used to predict miRNAs targeting TGFBR1, and miR-181a was selected as a candidate. To investigate the functions of miR-181a, miRNA mimics and inhibitors were used to overexpress or knockdown miR-181a, respectively. RT-qPCR and Western blotting were used to measure the expression of aP2, PPARγ, C/EBPα and TGFBR1 in porcine preadipocytes. Lipid accumulation and adipocyte apoptosis were detected using Oil Red O staining and flow cytometry, respectively. Taken together, our results indicated that miR-181a promoted porcine preadipocyte differentiation by directly targeting TGFBR1. Highlights • TGFBR1 is a target gene of miR-181a in porcine preadipocyte. • miR-181a reduced the mRNA and protein expression levels of TGFBR1. • miR-181a positively regulated porcine preadipocyte differentiation. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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