1. Cloning, expression and cellular localization of the Doublesex gene in the water flea, Daphnia carinata, during different developmental stages.
- Author
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Zhang M, Li H, Liu A, Wu D, Wang D, and Zhao Y
- Subjects
- Amino Acid Sequence, Animals, Base Sequence, Cloning, Molecular, DNA-Binding Proteins genetics, Daphnia metabolism, Drosophila Proteins genetics, Female, Gene Expression Regulation, Developmental, Molecular Sequence Data, Phylogeny, Reproduction genetics, Sequence Homology, Sex Determination Processes genetics, Tissue Distribution, Arthropod Proteins genetics, Arthropod Proteins metabolism, Daphnia genetics, Daphnia growth & development
- Abstract
In this study, one of Doublesex genes from the common freshwater cladoceran Daphnia carinata, designated DapcaDsx1, was cloned using primers based on homologous sequences and rapid amplification of cDNA ends (RACE). qPCR was employed to quantify differences in DapcaDsx1 expression between the different sexual phases, with expression levels being higher in sexual females. The role of DapcaDsx1 in the reproductive transformation was further investigated in parthenogenetic-phase females and sexual-phase females using whole-mount in situ hybridization. This cellular localization study showed specific expression of DapcaDsx1 in the thoracic segments, second antenna and part of the ventral carapace. Higher expression levels were exhibited in sexual females compared to parthenogenetic females. This suggests that the DapcaDsx1 gene plays significant roles in switching modes of reproduction and during sexual differentiation., (Copyright © 2014 Elsevier B.V. All rights reserved.)
- Published
- 2014
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