1. First report on delineation of differentially expressed genes and pathways in milk somatic cells of mastitic and healthy Murrah buffaloes.
- Author
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Ahlawat, Sonika, Choudhary, Vikas, Singh, Tersem, Arora, Reena, Kumar, Ashish, Kaur, Mandeep, Chhabra, Pooja, Sharma, Rekha, and Kumar Vijh, Ramesh
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MASTITIS , *SOMATIC cells , *MILK proteins , *REGULATOR genes , *GENES , *BOVINE mastitis , *GENETIC code , *GENE expression profiling - Abstract
• The transcriptional landscape of milk somatic cells of Murrah buffaloes with clinical mastitis was compared with the healthy controls. • A total of 1014 up-regulated and 999 down-regulated genes were observed in the diseased buffaloes (Fold change > 2, FDR < 0.05). • The expression of major milk protein genes (CSN2, CSN1S1, CSN1S2, PAEP and LALBA) was higher in the healthy buffaloes. • Mastitic buffaloes had higher transcript abundance of many genes coding for anti-microbial proteins. Despite immense contribution of buffaloes as dairy species, limited studies have addressed the bubaline mastitis as compared to cattle. This was the first differential transcriptomic study investigating the alterations induced by clinical mastitis in buffalo milk relative to healthy controls. Comparative gene expression profiling of three biological replicates of each group identified 1014 up-regulated and 999 down-regulated genes in the diseased buffaloes (Fold change > 2, FDR < 0.05). Activation of immune and inflammatory responses were the most enriched GO terms in the mastitic animals, with higher transcript abundance of many genes coding for anti-microbial proteins such as β-defensins, perforin, granzymes, granulysin, cathelicidins etc. Analysis of the gene regulatory interactions of the up-regulated DEGs identified many hub genes that govern the cellular and macromolecular metabolic processes (E2F4, E2F1, RBL2, FOXM1, IRF1 and MYB). This study contributes to an insightful understanding of molecular mechanisms governing immune response of buffaloes to mastitis. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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