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1. Isolation of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase

Author

Francis Karst and Ahmad Oulmouden

Subjects
biology, Genes, Fungal, Phosphotransferases, Saccharomyces cerevisiae, Mevalonate kinase, General Medicine, biology.organism_classification, MAP3K7, Molecular biology, Complementation, Phosphotransferases (Alcohol Group Acceptor), Gene expression, Genetics, Spore germination, biology.protein, Cloning, Molecular, Cyclin-dependent kinase 7, Gene, Plasmids
Abstract

The ERG12 gene of Saccharomyces cerevisiae has been cloned by complementation of an erg12-1 mutation affecting mevalonate kinase. From the 2.8-kb insert isolated, the functional gene has been localized on a DNA fragment of 2.1 kb. The mRNA is 1.45 kb long. Gene disruption shows that the ERG12 gene is essential in yeast, both for spore germination and vegetative growth.

Published
1990
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