1. Characterization of paralogous uncx transcription factor encoding genes in zebrafish
- Author
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Marcella Fiengo, Rita Marino, Takashi Kondo, Sylvie Maiella, Antonio Emidio Fortunato, Fernanda Langellotto, Raffaella De Paolo, Stephen W. Wilson, Giulia Fasano, Filomena Ristoratore, Lorella M.T. Canzoniero, Valeria Nittoli, Alessandra Gentile, Paolo Sordino, Ugo Coppola, Francesco Aniello, Aldo Donizetti, Denis Duboule, Immacolata Porreca, Nittoli, V., Fortunato, A. E., Fasano, G., Coppola, U., Gentile, A., Maiella, S., Langellotto, F., Porreca, I., De Paolo, R., Marino, R., Fiengo, M., Donizetti, A., Aniello, F., Kondo, T., Ristoratore, F., Canzoniero, L. M. T., Duboule, D., Wilson, S. W., and Sordino, P.
- Subjects
0301 basic medicine ,Ce, cerebellum ,Mutant ,CNE, conserved non-coding elements ,OP, olfactory placode ,HM, hybridization mix ,0302 clinical medicine ,CaP, caudal primary motor neuron axons ,VLP, ventro-lateral-posterior ,Ey, eye ,Gene duplication ,No, notochord ,fss, fused-somites ,TSGD ,FB, forebrain ,Zebrafish ,OT, optic tectum ,CRM, cis-regulatory module ,Di, diencephalon ,CAMP, conserved ancestral microsyntenic pairs ,Signaling pathway ,Ace, acerebellar ,PSM, presomitic mesoderm ,CS, Corpuscle of Stannius ,General Medicine ,YE, yolk extension ,Cell biology ,ptc, patched ,Te, telencephalon ,030220 oncology & carcinogenesis ,PA, pharyngeal arches ,embryonic structures ,Shh, sonic hedgehog ,syu, sonic-you ,WISH, whole-mount in situ hybridization ,yot, you-too ,HB, hindbrain ,Hy, hypothalamus ,animal structures ,lcsh:QH426-470 ,SC, spinal cord ,MO, morpholino ,Development ,Biology ,Elfn1, Extracellular Leucine Rich Repeat And Fibronectin Type III Domain Containing 1 ,Synteny ,Uncx ,Article ,03 medical and health sciences ,Flh, floating head ,Mical, molecule interacting with CasL ,Th, thalamus ,Yo, yolk ,Genetics ,Gene ,Transcription factor ,Messenger RNA ,fungi ,biology.organism_classification ,cyc, cyclops ,FGF, fibroblast growth factor ,lcsh:Genetics ,smu, slow-muscle-omitted ,030104 developmental biology ,TSGD, teleost-specific genome duplication ,So, somites ,Homeobox ,AP, antero-posterior ,hpf, hours post fertilization ,WIHC, whole-mount immunohistochemistry - Abstract
The paired-type homeodomain transcription factor Uncx is involved in multiple processes of embryogenesis in vertebrates. Reasoning that zebrafish genes uncx4.1 and uncx are orthologs of mouse Uncx, we studied their genomic environment and developmental expression. Evolutionary analyses indicate the zebrafish uncx genes as being paralogs deriving from teleost-specific whole-genome duplication. Whole-mount in situ mRNA hybridization of uncx transcripts in zebrafish embryos reveals novel expression domains, confirms those previously known, and suggests sub-functionalization of paralogs. Using genetic mutants and pharmacological inhibitors, we investigate the role of signaling pathways on the expression of zebrafish uncx genes in developing somites. In identifying putative functional role(s) of zebrafish uncx genes, we hypothesized that they encode transcription factors that coordinate growth and innervation of somitic muscles., Highlights • The Uncx4.1 and Uncx genes derive from the teleost-specific whole-genome duplication. • Uncx genes are expressed during embryogenesis in unique and overlapping domains. • Uncx gene expression during somite differentiation is regulated by FGF signaling. • Synteny and expression profiles correlate Uncx genes with axon guidance.
- Published
- 2019