1. Stress-induced expression of a novel variant of human fumarate hydratase (FH).
- Author
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Lehtonen HJ, Ylisaukko-Oja SK, Kiuru M, Karhu A, Lehtonen R, Vanharanta S, Jalanko A, Aaltonen LA, and Launonen V
- Subjects
- Base Sequence, Blotting, Western, Cell Line, DNA, Complementary, Fluorescent Antibody Technique, Humans, Molecular Sequence Data, Protein Biosynthesis, Subcellular Fractions enzymology, Fumarate Hydratase genetics, Gene Expression Regulation, Stress, Physiological genetics
- Abstract
Fumarate hydratase (FH) is an enzyme of the mitochondrial tricarboxylic acid cycle (TCAC). Here we report the characterization of a novel FH variant (FHv) that contains an alternative exon 1b, thus lacking the mitochondrial signal sequence. Distinct from mitochondrial FH, FHv localized to cytosol and nucleus and lacked FH enzyme activity. FHv was expressed ubiquitously in human fetal and adult tissues. Heat shock and prolonged hypoxia increased FHv expression in a cell line (HTB 115) by nine- and fourfold, respectively. These results suggest that FHv has an alternative function outside the TCAC related to cellular stress response.
- Published
- 2007
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