1. Isolation and molecular identification of two novel cyanobacterial isolates obtained from a stressed aquatic system
- Author
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Mahmoud M. A. Moustafa, Hoda A.S. El-Garhy, Ibrahim I.S. EL-Shawaf, and Omnia A. M. Badr
- Subjects
0301 basic medicine ,Cyanobacteria ,chemistry.chemical_classification ,biology ,Phylogenetic tree ,biology.organism_classification ,16S ribosomal RNA ,Isolation (microbiology) ,03 medical and health sciences ,030104 developmental biology ,chemistry ,Botany ,Genetics ,Clade ,Gene ,Carotenoid ,Molecular identification - Abstract
Cyanobacteria are immense sources of several pharmacological compounds such as flavonoids and carotenoids with anti-inflammatory and antioxidant activity. Two novel cyanobacterial isolates were isolated and identified by sequencing of 16S rRNA gene. The results revealed that the two isolates are Sphaerospermopsis aphanizomenoides (KU212886.1) and Cronbergia siamensis (KY296358.1). The BLAST alignment showed that the nearest NCBI deposited sequences were Cronbergia siamensis (NR153750.1) to C. siamensis (KY296358.1) and Sphaerospermopsis aphanizomenoides (LN846950.1) to S. aphanizomenoides (KU212886.1) with identity ratio 94% and 93%, respectively. The phylogenetic trees confirmed the same identity ratios on the roots of clades. The two newly identified isolates could be new species with novel and unique characteristics.
- Published
- 2018
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