1. Establishment of a novel human myeloid leukemia cell line, AMU-AML1, carrying t(12;22)(p13;q11) without chimeric MN1-TEL and with high expression of MN1
- Author
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Hidetsugu Mihara, Masafumi Taniwaki, Hidesuke Yamamoto, Mayuko Gotou, Kazuto Suganuma, Tomohiro Horio, Akihito Hiramatsu, Masato Shikami, Motohiro Wakabayashi, Masaya Watarai, Ichiro Hanamura, Hiroshi Miwa, Miyuki Takahashi, Tomohiko Taki, Shohei Mizuno, Norikazu Tsunekawa, Natsumi Sakamoto, Masakazu Nitta, Mineaki Goto, Hisao Nagoshi, and Akira Imamura
- Subjects
Untranslated region ,Male ,Cancer Research ,Oncogene Proteins, Fusion ,Transcription, Genetic ,Chromosomes, Human, Pair 22 ,CD33 ,Gene Expression ,Biology ,Translocation, Genetic ,Immunophenotyping ,Chromosome Breakpoints ,hemic and lymphatic diseases ,Cell Line, Tumor ,Gene expression ,Gene Order ,Genetics ,medicine ,Humans ,In Situ Hybridization, Fluorescence ,Regulation of gene expression ,Comparative Genomic Hybridization ,Chromosomes, Human, Pair 12 ,Gene Expression Regulation, Leukemic ,Tumor Suppressor Proteins ,Spectral Karyotyping ,Myeloid leukemia ,Middle Aged ,medicine.disease ,Molecular biology ,Chromosome Banding ,Leukemia ,Leukemia, Myeloid ,Trans-Activators ,Comparative genomic hybridization ,Transcription Factors - Abstract
In this study, we established and analyzed a novel human myeloid leukemia cell line, AMU-AML1, from a patient with acute myeloid leukemia with multilineage dysplasia before the initiation of chemotherapy. AMU-AML1 cells were positive for CD13, CD33, CD117, and HLA-DR by flow cytometry analysis and showed a single chromosomal abnormality, 46, XY, t(12;22)(p13;q11.2), by G-banding and spectral karyotyping. Fluorescent in situ hybridization analysis indicated that the chromosomal breakpoint in band 12p13 was in the sequence from the 5' untranslated region to intron 1 of TEL and that the chromosomal breakpoint in band 22q11 was in the 3' untranslated region of MN1. The chimeric transcript and protein of MN1-TEL could not be detected by reverse-transcriptase polymerase chain reaction or Western blot analysis. However, the MN1 gene was amplified to three copies detected by array comparative genomic hybridization analysis, and the expression levels of the MN1 transcript and protein were high in AMU-AML1 cells when compared with other cell lines with t(12;22)(p13;q11-12). Our data showed that AMU-AML1 cells contain t(12;22)(p13;q11.2) without chimeric fusion of MN1 and TEL. The AMU-AML1 cells gained MN1 copies and had high expression levels of MN1. Thus, the AMU-AML1 cell line is useful for studying the biological consequences of t(12;22)(p13;q11.2) lacking chimeric MN1-TEL.
- Published
- 2011