1. State-dependent Ras signaling and AMPA receptor trafficking.
- Author
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Yi Qin, Yinghua Zhu, Baumgart, Joel P., Stornetta, Ruth L., Seidenman, Kenneth, Mack, Volker, Van Aelst, Linda, and Zhu, J. Julius
- Subjects
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GUANOSINE triphosphatase , *GTPASE-activating protein , *NEUROPLASTICITY , *RENIN-angiotensin system , *NEUROPHYSIOLOGY , *PROTEIN kinases - Abstract
Synaptic trafficking of AMPA-Rs, controlled by small GTPase Ras signaling, plays a key role in synaptic plasticity. However, how Ras signals synaptic AMPA-R trafficking is unknown. Here we show that low levels of Ras activity stimulate extracellular signal-regulated kinase kinase (MEK)-p42/44 MAPK (extracellular signal-regulated kinase [ERK]) signaling, whereas high levels of Ras activity stimulate additional Pi3 kinase (Pi3K)-protein kinase B (PKB) signaling, each accounting for ∼50% of the potentiation during long-term potentiation (LTP). Spontaneous neural activity stimulates the Ras-MEK-ERK pathway that drives GluR2L into synapses. In the presence of neuromodulator agonists, neural activity also stimulates the Ras-Pi3K-PKB pathway that drives GluR1 into synapses. Neuromodulator release increases with increases of vigilance. Correspondingly, Ras-MEK-ERK activity in sleeping animals is sufficient to deliver GluR2L into synapses, while additional increased Ras-Pi3K-PKB activity in awake animals delivers GluR1 into synapses. Thus, state-dependent Ras signaling, which specifies downstream MEK-ERK and Pi3K-PKB pathways, differentially control GluR2L- and GluR1-dependent synaptic plasticity. [ABSTRACT FROM AUTHOR]
- Published
- 2005
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