1. Predicting the response of the dental pulp to SARS-CoV2 infection: a transcriptome-wide effect cross-analysis
- Author
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Pietro Hiram Guzzi, Federico M. Giorgi, Johnah C. Galicia, Asma A. Khan, Galicia J.C., Guzzi P.H., Giorgi F.M., and Khan A.A.
- Subjects
0301 basic medicine ,Datasets as Topic ,Disease ,Pathogenesis ,Transcriptome ,0302 clinical medicine ,Receptors, Viru ,Genetics(clinical) ,Pulpitis ,skin and connective tissue diseases ,Genetics (clinical) ,Regulation of gene expression ,Serine Endopeptidases ,Receptors, Virus ,Angiotensin-Converting Enzyme 2 ,medicine.symptom ,Coronavirus Infections ,Infection ,Pulpiti ,Human ,Pneumonia, Viral ,Immunology ,Inflammation ,Peptidyl-Dipeptidase A ,Biology ,Brief Communication ,Betacoronavirus ,03 medical and health sciences ,stomatognathic system ,Human interactome ,Genetics ,medicine ,Humans ,Pandemics ,Dental Pulp ,Betacoronaviru ,Pandemic ,Coronavirus Infection ,SARS-CoV-2 ,Gene Expression Profiling ,COVID-19 ,Receptors, Coronaviru ,medicine.disease ,Gene expression profiling ,stomatognathic diseases ,030104 developmental biology ,Gene Expression Regulation ,Gene expression ,Receptors, Coronavirus ,030215 immunology - Abstract
Pulpitis, inflammation of the dental pulp, is a disease that often necessitates emergency dental care. While pulpitis is considered to be a microbial disease primarily caused by bacteria, viruses have also been implicated in its pathogenesis. Here, we determined the expression of the SARS-CoV2 receptor, angiotensin converting enzyme 2 (ACE2) and its associated cellular serine protease TPMRSS2 in the dental pulp under normal and inflamed conditions. Next, we explored the relationship between the SARS-CoV-2/human interactome and genes expressed in pulpitis. Using existing datasets we show that both ACE2 and TPMRSS2 are expressed in the dental pulp and, that their expression does not change under conditions of inflammation. Furthermore, Master Regulator Analysis of the SARS-CoV2/human interactome identified 75 relevant genes whose expression values are either up-regulated or down-regulated in both the human interactome and pulpitis. Our results suggest that the dental pulp is vulnerable to SARS-CoV2 infection and that SARS-CoV-2 infection of the dental pulp may contribute to worse outcomes of pulpitis.
- Published
- 2020
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