1. Chromatin-to-nucleoprotamine transition is controlled by the histone H2B variant TH2B
- Author
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Sophie Barral, Emilie Montellier, Alexandra Debernardi, Fabrice Lopez, Philippe Guardiola, Sandrine Curtet, Carlo Petosa, Hélène Holota, Minjia Tan, Jean Imbert, Karin Pernet, Sophie Rousseaux, Patrick Héry, Matthieu Gérard, Kai Zhang, Mahya Jamshidikia, Saadi Khochbin, Yingming Zhao, Hitoshi Shiota, François Fenaille, Fayçal Boussouar, Aurélie Bergon, Thierry Buchou, Laboratoire Adaptation et pathogénie des micro-organismes [Grenoble] (LAPM), Université Joseph Fourier - Grenoble 1 (UJF)-Centre National de la Recherche Scientifique (CNRS), Institut de biologie structurale (IBS - UMR 5075 ), Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institute for Advanced Biosciences / Institut pour l'Avancée des Biosciences (Grenoble) (IAB), Centre Hospitalier Universitaire [Grenoble] (CHU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Etablissement français du sang - Auvergne-Rhône-Alpes (EFS)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes [2016-2019] (UGA [2016-2019]), Université Grenoble Alpes [2016-2019] (UGA [2016-2019])-Institut de Recherche Interdisciplinaire de Grenoble (IRIG), and Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Centre National de la Recherche Scientifique (CNRS)
- Subjects
Male ,Epigenesis, Genetic ,Histones ,Mice ,0302 clinical medicine ,MESH: Gene Expression Regulation, Developmental ,Histone methylation ,Testis ,Histone code ,MESH: Animals ,MESH: Epigenesis, Genetic ,Protamines ,MESH: Histones ,Genetics ,0303 health sciences ,Genome ,[SDV.BBM.BS]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Structural Biology [q-bio.BM] ,MESH: Testis ,Gene Expression Regulation, Developmental ,Chromatin ,Cell biology ,Nucleosomes ,Meiosis ,030220 oncology & carcinogenesis ,Histone methyltransferase ,Female ,MESH: Spermatogenesis ,endocrine system ,Biology ,Chromatin remodeling ,MESH: Chromatin ,03 medical and health sciences ,Histone H1 ,MESH: Nucleosomes ,Histone H2A ,Histone H2B ,Nucleosome ,Animals ,MESH: Genome ,Spermatogenesis ,MESH: Mice ,030304 developmental biology ,Extra View ,MESH: Protamines ,MESH: Male ,MESH: Meiosis ,Fertilization ,MESH: Fertilization ,MESH: Female ,Developmental Biology - Abstract
International audience; The conversion of male germ cell chromatin to a nucleoprotamine structure is fundamental to the life cycle, yet the underlying molecular details remain obscure. Here we show that an essential step is the genome-wide incorporation of TH2B, a histone H2B variant of hitherto unknown function. Using mouse models in which TH2B is depleted or C-terminally modified, we show that TH2B directs the final transformation of dissociating nucleosomes into protamine-packed structures. Depletion of TH2B induces compensatory mechanisms that permit histone removal by up-regulating H2B and programming nucleosome instability through targeted histone modifications, including lysine crotonylation and arginine methylation. Furthermore, after fertilization, TH2B reassembles onto the male genome during protamine-to-histone exchange. Thus, TH2B is a unique histone variant that plays a key role in the histone-to-protamine packing of the male genome and guides genome-wide chromatin transitions that both precede and follow transmission of the male genome to the egg.
- Published
- 2013