1. The Dlk1 and Gtl2 genes are linked and reciprocally imprinted
- Author
-
J V, Schmidt, P G, Matteson, B K, Jones, X J, Guan, and S M, Tilghman
- Subjects
Muridae ,Genomic Imprinting ,Research Communication ,Peromyscus ,Base Sequence ,Genetic Linkage ,Reverse Transcriptase Polymerase Chain Reaction ,Animals ,Chromosome Mapping ,Gene Expression Regulation, Developmental ,Female ,DNA Methylation ,DNA Primers - Abstract
Genes subject to genomic imprinting exist in large chromosomal domains, probably reflecting coordinate regulation of the genes within a cluster. Such regulation has been demonstrated for the H19, Igf2, and Ins2 genes that share a bifunctional imprinting control region. We have identified the Dlk1 gene as a new imprinted gene that is paternally expressed. Furthermore, we show that Dlk1 is tightly linked to the maternally expressed Gtl2 gene. Dlk1 and Gtl2 are coexpressed and respond in a reciprocal manner to loss of DNA methylation. These genes are likely to represent a new example of coordinated imprinting of linked genes.
- Published
- 2000