1. High-density linkage maps and sex-linked markers for the black tiger shrimp (Penaeus monodon)
- Author
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Staelens, Jan, Rombaut, Debbie, Vercauteren, Ilse, Argue, Brad, Benzie, John, and Vuylsteke, Marnik
- Subjects
Shrimps -- Genetic aspects ,Linkage (Genetics) -- Evaluation ,Genetic markers -- Observations ,Chromosome mapping -- Methods ,Biological sciences - Abstract
We report on the construction of sex-specific high-density linkage maps and identification of sex-linked markers for the black tiger shrimp (Penaeus monodon). Overall, we identified 44 male and 43 female linkage groups (2n = 88) from the analysis of 2306 AFLP markers segregating in three full-sib families, covering 2378 and 2362 cM, respectively. Twenty-one putatively homologous linkage groups, including the sex-linkage groups, were identified between the female and male linkage maps. Six sex-linked AFLP marker alleles were inherited from female parents in the three families, suggesting that the P. monodon adopts a WZ-ZZ sex-determining system. Two sex-linked AFLP markers, one of which we converted into an allele-specific assay, confirmed their association with sex in a panel of 52 genetically unrelated animals.
- Published
- 2008