1. Dietary glycerol for quail: association between productive performance and COX III mRNA expression.
- Author
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Silva SC, Gasparino E, Batista E, Tanamati F, Vesco AP, Lala B, and de Oliveira DP
- Subjects
- Animal Feed, Animals, Diet, Electron Transport Complex IV genetics, Gene Expression Regulation drug effects, Muscle, Skeletal growth & development, Muscle, Skeletal metabolism, Quail metabolism, RNA, Messenger biosynthesis, RNA, Messenger genetics, Electron Transport Complex IV biosynthesis, Glycerol administration & dosage, Muscle, Skeletal drug effects, Quail genetics
- Abstract
This study was carry out to evaluate mRNA expression of mitochondrial cytochrome c oxidase III in the Pectoralis superficialis muscle of 28-day-old quails fed diets containing 0, 8, and 12% glycerol. Total RNA was extracted (N = 10) and cDNA was amplified using specifics primers for qRT-PCR. Feed efficiency and feed intake were evaluated. COX III mRNA expression in breast muscle was higher in the group fed with 12% glycerol (0.863 AU); no differences were observed in the expression of this gene between the muscle of animals fed diets without glycerol (0.357 AU) and 8% glycerol (0.415 AU). Quails that showed greater COX III mRNA expression also showed the lowest feed efficiency. These results show that there is a difference in COX III mRNA expression in breast muscle of 28-day-old quail fed diets different concentrations of glycerol.
- Published
- 2016
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