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Your search keyword '"Andrew Zimmer"' showing total 2 results
Start Over Author "Andrew Zimmer" Journal genome biology
2 results on '"Andrew Zimmer"'

1. A scalable, fully automated process for construction of sequence-ready human exome targeted capture libraries

Author

Frank Juhn, Jillian Nolan, Chad Nusbaum, Robert Nicol, Alexander Allen, Geneva Young, Aaron M. Berlin, Andrew Barry, John Stalker, Sean M. Sykes, Jon Thompson, John Jarlath Walsh, Andrew Zimmer, Toni Delorey, Justin Abreu, Ramy Shammas, Sheila Fisher, Brian Minie, Lauren Ambrogio, Kristian Cibulskis, Zac Zwirko, Ryan Johnson, Dennis C. Friedrich, Brendan Blumenstiel, Timothy Fennell, Brian Reilly, and Stacey Gabriel

Subjects
Quality Control, Process (engineering), Method, Biology, Genome, 03 medical and health sciences, 0302 clinical medicine, Humans, Exome, Protocol (object-oriented programming), Throughput (business), 030304 developmental biology, Gene Library, Oligonucleotide Array Sequence Analysis, Genetics, Automation, Laboratory, 0303 health sciences, business.industry, Genome, Human, High-Throughput Nucleotide Sequencing, Nucleic Acid Hybridization, Automation, Computer architecture, 030220 oncology & carcinogenesis, Scalability, Human genome, business
Abstract

Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.

Published
2010

2. A scalable, fully automated process for construction of sequence-ready barcoded libraries for 454

Author

Andrew Barry, Pablo Alvarez, Danielle Perrin, Matthew R. Henn, William Brockman, Georgia Giannoukos, Chad Nusbaum, Andrew Hollinger, Scott Anderson, Dana Robbins, Michael Weiand, Robert Nicol, Todd Sparrow, Taryn L Powers, David B. Jaffe, Scott Steelman, Frank Juhn, Cindi A. Hoover, Carsten Russ, Kamran Rizzolo, Andrew Zimmer, Danielle McCarthy, Robert E. Lintner, Elizabeth M. Ryan, John Stalker, Niall J. Lennon, Lisa Green, Riza M. Daza, Rachel L. Erlich, and Karen Ponchner

Subjects
Sample handling, Genetics, Electronic Data Processing, Sequence, Process (engineering), business.industry, Method, Sequence Analysis, DNA, Biology, Microspheres, High-Throughput Screening Assays, Fully automated, Embedded system, Magnetic bead, Scalability, Humans, Sources of error, business, Throughput (business), Algorithms, Gene Library
Abstract

An automated method for constructing libraries for 454 sequencing significantly reduces the cost and time required., We present an automated, high throughput library construction process for 454 technology. Sample handling errors and cross-contamination are minimized via end-to-end barcoding of plasticware, along with molecular DNA barcoding of constructs. Automation-friendly magnetic bead-based size selection and cleanup steps have been devised, eliminating major bottlenecks and significant sources of error. Using this methodology, one technician can create 96 sequence-ready 454 libraries in 2 days, a dramatic improvement over the standard method.

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