1. Minimal genome-wide human CRISPR-Cas9 library.
- Author
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Gonçalves E, Thomas M, Behan FM, Picco G, Pacini C, Allen F, Vinceti A, Sharma M, Jackson DA, Price S, Beaver CM, Dovey O, Parry-Smith D, Iorio F, Parts L, Yusa K, and Garnett MJ
- Subjects
- Gene Library, Genome-Wide Association Study, Humans, Organoids, RNA, Guide, CRISPR-Cas Systems genetics, CRISPR-Cas Systems, Genome, Human, Genomic Library
- Abstract
CRISPR guide RNA libraries have been iteratively improved to provide increasingly efficient reagents, although their large size is a barrier for many applications. We design an optimised minimal genome-wide human CRISPR-Cas9 library (MinLibCas9) by mining existing large-scale gene loss-of-function datasets, resulting in a greater than 42% reduction in size compared to other CRISPR-Cas9 libraries while preserving assay sensitivity and specificity. MinLibCas9 provides backward compatibility with existing datasets, increases the dynamic range of CRISPR-Cas9 screens and extends their application to complex models and assays.
- Published
- 2021
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