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A novel approach to prepare 3' end fragments from polyadenylated RNA to map the position of the poly (A) addition site using oligonucleotide arrays tiling 1 percent of the human genome and gain insight into transcript 3' end formation and transcription termination. Results provide insight into extensive sites of poly (A) addition and relatively complex mechanism of transcription termination that involves reduction of histone 3 lysine 36 trimethylation prior to RNA polymerase dephosphorylation and release from DNA.

The transcription factor binding region mapping is done by using chromatin immunoprecipitation (Chip) technique in DNA microarray analysis and in DNA sequencing. Both the techniques were found to be complementary to each other in their detection capacities.