1. Microarray analysis of gene expression profile in resistant and susceptible Bombyx mori strains reveals resistance-related genes to nucleopolyhedrovirus.
- Author
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Zhou, Yang, Gao, Lu, Shi, Haifeng, Xia, Hengchuan, Gao, Li, Lian, Chaoqun, Chen, Liang, Yao, Qin, Chen, Keping, and Liu, Xiaoyong
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PROTEIN microarrays , *GENE expression in plants , *SILKWORMS , *NUCLEOPOLYHEDROVIRUSES , *MOLECULAR biology , *POLYMERASE chain reaction , *IMMUNE response - Abstract
Abstract: To investigate the molecular mechanism of silkworm resistance to BmNPV infection, we constructed a near-isogenic line (BC8) with BmNPV resistance using highly resistant (NB) and highly susceptible parental strains (306). We investigated variations in the gene expression in the midguts of BmNPV-infected BC8 and 306 at 12h pi using the microarray. 92 differentially expressed genes were identified. Real-time qPCR analysis confirmed that 10 genes were significantly up-regulated or down-regulated in the midguts of BC8 and NB compared to 306. To our knowledge, we first defined the role of the amino acid transporter and 26S proteasome in insect antiviral. However, serine protease was not completely consistent with data of reported previously in insect antiviral. The role of the 5 genes (Bm123, Bm122, COP β′, aquaporin, glycoside hydrolases) was also demonstrated in insect antiviral. Our results provided new insights into the molecular mechanism of the Bombyx mori immune response against BmNPV infection. [Copyright &y& Elsevier]
- Published
- 2013
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