1. Structure of the human type I iodothyronine 5'-deiodinase gene and localization to chromosome 1p32-p33.
- Author
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Jakobs TC, Koehler MR, Schmutzler C, Glaser F, Schmid M, and Köhrle J
- Subjects
- Base Sequence, Chromosome Mapping, Cloning, Molecular, Cosmids, DNA Primers genetics, Exons, Gene Library, Humans, In Situ Hybridization, Fluorescence, Introns, Iodide Peroxidase classification, Polymerase Chain Reaction, Restriction Mapping, Chromosomes, Human, Pair 1 genetics, Iodide Peroxidase genetics
- Abstract
The human type I iodothyronine 5'-deiodinase gene encodes a member of the family of selenocysteine-containing deiodinases. These enzymes catalyze the activation of the prohormone thyroxine to 3,3',5-triiodothyronine or the degradation of thyroxine and triiodothyronine to inactive metabolites. Here we report the isolation of two genomic type I 5'-deiodinase clones from a chromosome 1-specific gridded cosmid library, the localization of the gene to chromosome 1p32-p33 by fluorescence in situ hybridization, and the determination of the complete structure of the 17.5-kb gene.
- Published
- 1997
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