1. Functional evaluation of human papillomavirus type 31 long control region variants
- Author
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Rita de Cássia Pereira de Lima, Ana Pavla Almeida Diniz Gurgel, R. C. O. Silva, Antonio Carlos de Freitas, M. N. Cordeiro, Lindomar Pena, Marcus Vinicius de Aragão Batista, D. L. Santos, and Bárbara Simas Chagas
- Subjects
0106 biological sciences ,Transcriptional Activation ,Biology ,01 natural sciences ,03 medical and health sciences ,Viral Proteins ,Gene expression ,Genetics ,Humans ,Luciferase ,Human papillomavirus 31 ,Promoter Regions, Genetic ,030304 developmental biology ,Cloning ,0303 health sciences ,Reporter gene ,Functional evaluation ,Polymorphism, Genetic ,virus diseases ,Promoter ,Transfection ,Molecular biology ,female genital diseases and pregnancy complications ,DNA-Binding Proteins ,Subcloning ,Trans-Activators ,010606 plant biology & botany ,HeLa Cells - Abstract
Persistent infections by high-risk human papillomavirus (HR-HPV) are a necessary condition, but not sufficient for cervical cancer development. Genetic variants of HR-HPV appear to be related to the risk of persistent infections. The study performed a functional evaluation of variants of the HPV-31 promoter region (LCR). For this, cloning and subcloning of variants HPV-31/UFPE-21 HPV-31/UFPE-89, HPV-31/UFPE-66, E2 gene and prototype HPV-31 were performed. Transfection with different concentrations of E2 was done and the concentration of 25 ng was determined to be ideal for LCR activation. HPV-31/UFPE-21 and HPV-31/UFPE-89 have a greater ability to alter Nluc reporter gene expression levels and HPV-31/UFPE-66 showed decreased levels of gene expression of Nluc reporter gene compared to control. Statistical analysis showed a significant difference between the polymorphic LCR regions and the control (p 0.0001). A more refined profile of variants of HPV-31 and its importance for the prognosis of cervical lesions begins to be drawn.
- Published
- 2020