1. Difference in number of loci of swine leukocyte antigen classical class I genes among haplotypes.
- Author
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Tanaka-Matsuda M, Ando A, Rogel-Gaillard C, Chardon P, and Uenishi H
- Subjects
- Amino Acid Sequence, Animals, Chromosomes, Artificial, Bacterial, Gene Duplication, Gene Expression Regulation, Haplotypes, Histocompatibility Antigens Class I chemistry, Histocompatibility Antigens Class I metabolism, Histocompatibility Antigens Class II, Molecular Sequence Data, Phylogeny, Swine immunology, Genes, MHC Class I genetics, Genetic Variation, Histocompatibility Antigens Class I genetics, Swine genetics
- Abstract
The structure of the entire genomic region of swine leukocyte antigen (SLA)-the porcine major histocompatibility complex--was recently elucidated in a particular haplotype named Hp-1.0 (H01). However, it has been suggested that there are differences in the number of loci of SLA genes, particularly classical class I genes, among haplotypes. To clarify the between-haplotype copy number variance in genes of the SLA region, we sequenced the genomic region carrying SLA classical class I genes on two different haplotypes, revealing increments of up to six in the number of classical class I genes in a single haplotype. All of the SLA-1(-like) (SLA-1 and newly designated SLA-12) and SLA-3 genes detected in the haplotypes thus analyzed were transcribed in the individual. The process by which duplication of SLA classical class I genes was likely to have occurred was interpreted from an analysis of repetitive sequences adjacent to the duplicated class I genes.
- Published
- 2009
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